|
| Status |
Public on Aug 01, 2015 |
| Title |
L1 DD, repeat 4 |
| Sample type |
RNA |
| |
|
| Source name |
DD neurons from L1 larvae
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
tissue: DD motor neurons
age: L1 larva
genotype: CZ8332 [juIs223 (pttr-39::mcherry, pttx-3::GFP) IV]
label: GFP
|
| Treatment protocol |
Embryos were prepared by standard bleach-prep protocol and allowed to hatch overnight in M9 buffer at 20 C to produce a population of growth-arrested L1 stage larvae.
|
| Growth protocol |
Nematodes were grown on bacterial strain NA22.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from Trizol and DNase-treated using Zymo’s RNA cleanup kit: the “DNA-Free RNA Kit” (R1013) as described by the manufacturers.
|
| Label |
biotin
|
| Label protocol |
5-10 ng of total RNA was ampified using the NuGEN WT-Ovation Pico Amplification System. Amplified cDNA was fragmented and labeled according to manufacturer's instructions in NuGEN Biotin RNA Amplification and Labeling System.
|
| |
|
| Hybridization protocol |
Affymetrix
|
| Scan protocol |
Genechips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
L1 DD repeat 4
|
| Data processing |
Data were normalized using quantile normalization and Robust Multiarray Analysis (RMA) summarization available in RMAExpress.
|
| |
|
| Submission date |
Jul 31, 2015 |
| Last update date |
Aug 01, 2015 |
| Contact name |
Siwei He |
| E-mail(s) |
siwei.he@vanderbilt.edu
|
| Organization name |
Vanderbilt University
|
| Department |
Cell and Developmental Biology
|
| Lab |
Miller Lab
|
| Street address |
465 21st Ave South
|
| City |
Nasvhille |
| State/province |
TN |
| ZIP/Postal code |
37212 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE71618 |
Expression data from DD neurons isolated from early L1 stage C. elegans larvae. |
|
