gender: male sample type: cell line disease state: cancer cell line: HepG2 cell line
Growth protocol
HepG2, SKHep1 and MCF-7 cells were maintained in MEM medium (Gibco, Invitrogen, Life Technologies). MCF7 cells were cultured with 0.01 mg/ml of insulin (Invitrogen, Carlsbad, California). MDA-MB-231 cells were cultured in Dulbecco’s modified Eagle’s medium (Invitrogen). PC3 and LNCaP were cultured in RPMI1640 media (Gibco, Invitrogen, Life Technologies). All media were supplemented with 2 mmol/L glutamine (Sigma-Aldrich), 10% FBS (Gibco), 1 U/mL penicillin and 1 mg/mL streptomycin (Gibco).
Extracted molecule
total RNA
Extraction protocol
RNA was
Label
biotin
Label protocol
biotin-labeled cRNA using Illumina® TotalPrep RNA Amplification Kit, according to manufacturer’s instructions (Life Technologies)
Hybridization protocol
Standard Illumina hybridization protocol
Scan protocol
Standard Illumina scanning protocol
Description
replicate 2
Data processing
The data were normalised using quantile normalisation with Genome Studio expression module
A common promoter hypomethylation signature in invasive breast, liver and prostate cancer cell lines reveals novel targets involved in cancer invasiveness (expression)
A common promoter hypomethylation signature in invasive breast, liver and prostate cancer cell lines reveals novel targets involved in cancer invasiveness