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Sample GSM1841864 Query DataSets for GSM1841864
Status Public on Feb 09, 2017
Title HepG2 rep2
Sample type RNA
 
Source name HepG2 cell line
Organism Homo sapiens
Characteristics gender: male
sample type: cell line
disease state: cancer
cell line: HepG2 cell line
Growth protocol HepG2, SKHep1 and MCF-7 cells were maintained in MEM medium (Gibco, Invitrogen, Life Technologies). MCF7 cells were cultured with 0.01 mg/ml of insulin (Invitrogen, Carlsbad, California). MDA-MB-231 cells were cultured in Dulbecco’s modified Eagle’s medium (Invitrogen). PC3 and LNCaP were cultured in RPMI1640 media (Gibco, Invitrogen, Life Technologies). All media were supplemented with 2 mmol/L glutamine (Sigma-Aldrich), 10% FBS (Gibco), 1 U/mL penicillin and 1 mg/mL streptomycin (Gibco).
Extracted molecule total RNA
Extraction protocol RNA was
Label biotin
Label protocol biotin-labeled cRNA using Illumina® TotalPrep RNA Amplification Kit, according to manufacturer’s instructions (Life Technologies)
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 2
Data processing The data were normalised using quantile normalisation with Genome Studio expression module
 
Submission date Jul 31, 2015
Last update date Feb 09, 2017
Contact name David Cheishvili
E-mail(s) david.cheishvili@mcgill.ca
Phone 5143987107
Organization name McGill
Street address ​3655 Sir William Osler
City Montreal
State/province Quebec
ZIP/Postal code H3G 1Y6
Country Canada
 
Platform ID GPL10558
Series (2)
GSE71625 A common promoter hypomethylation signature in invasive breast, liver and prostate cancer cell lines reveals novel targets involved in cancer invasiveness (expression)
GSE71627 A common promoter hypomethylation signature in invasive breast, liver and prostate cancer cell lines reveals novel targets involved in cancer invasiveness

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 -6.076713 0.738961
ILMN_2055271 16.36185 0.04545455
ILMN_1736007 -5.811965 0.7272727
ILMN_2383229 1039.617 0
ILMN_1806310 1342.805 0
ILMN_1779670 35.44302 0.001298701
ILMN_1653355 6.691503 0.1922078
ILMN_1717783 -4.576333 0.6701299
ILMN_1705025 17.54252 0.03376623
ILMN_1814316 1.49764 0.3714286
ILMN_2359168 5.929698 0.2116883
ILMN_1731507 -4.947739 0.6857143
ILMN_1787689 0.7925578 0.4207792
ILMN_3241953 105.6884 0
ILMN_1745607 1891.505 0
ILMN_2136495 0.2444493 0.4467532
ILMN_1668111 -2.151587 0.5467532
ILMN_2295559 12.19912 0.08181818
ILMN_1735045 27.54676 0.007792208
ILMN_1680754 19.57193 0.02467532

Total number of rows: 47311

Table truncated, full table size 1421 Kbytes.




Supplementary file Size Download File type/resource
GSM1841864_8909358064_E_Grn.idat.gz 1.6 Mb (ftp)(http) IDAT
Processed data included within Sample table

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