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Sample GSM1841866 Query DataSets for GSM1841866
Status Public on Feb 09, 2017
Title SKHep1 rep1
Sample type RNA
 
Source name SKHep1 cell line
Organism Homo sapiens
Characteristics gender: male
sample type: cell line
disease state: cancer
cell line: SKHep1 cell line
Growth protocol HepG2, SKHep1 and MCF-7 cells were maintained in MEM medium (Gibco, Invitrogen, Life Technologies). MCF7 cells were cultured with 0.01 mg/ml of insulin (Invitrogen, Carlsbad, California). MDA-MB-231 cells were cultured in Dulbecco’s modified Eagle’s medium (Invitrogen). PC3 and LNCaP were cultured in RPMI1640 media (Gibco, Invitrogen, Life Technologies). All media were supplemented with 2 mmol/L glutamine (Sigma-Aldrich), 10% FBS (Gibco), 1 U/mL penicillin and 1 mg/mL streptomycin (Gibco).
Extracted molecule total RNA
Extraction protocol RNA was
Label biotin
Label protocol biotin-labeled cRNA using Illumina® TotalPrep RNA Amplification Kit, according to manufacturer’s instructions (Life Technologies)
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing The data were normalised using quantile normalisation with Genome Studio expression module
 
Submission date Jul 31, 2015
Last update date Feb 09, 2017
Contact name David Cheishvili
E-mail(s) david.cheishvili@mcgill.ca
Phone 5143987107
Organization name McGill
Street address ​3655 Sir William Osler
City Montreal
State/province Quebec
ZIP/Postal code H3G 1Y6
Country Canada
 
Platform ID GPL10558
Series (2)
GSE71625 A common promoter hypomethylation signature in invasive breast, liver and prostate cancer cell lines reveals novel targets involved in cancer invasiveness (expression)
GSE71627 A common promoter hypomethylation signature in invasive breast, liver and prostate cancer cell lines reveals novel targets involved in cancer invasiveness

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 -4.88283 0.6844156
ILMN_2055271 52.04268 0.001298701
ILMN_1736007 -2.10857 0.5350649
ILMN_2383229 2.51541 0.3636364
ILMN_1806310 -0.6378361 0.4792208
ILMN_1779670 7.514619 0.2064935
ILMN_1653355 3.90899 0.3272727
ILMN_1717783 -9.270477 0.8467532
ILMN_1705025 0.421101 0.4402598
ILMN_1814316 -5.49064 0.7116883
ILMN_2359168 -5.373256 0.7077922
ILMN_1731507 -9.50235 0.8571429
ILMN_1787689 7.729431 0.2
ILMN_3241953 153.4482 0
ILMN_1745607 -15.30424 0.9766234
ILMN_2136495 -6.122813 0.7402598
ILMN_1668111 -2.315134 0.5441558
ILMN_2295559 -8.468856 0.8220779
ILMN_1735045 36.19005 0.006493506
ILMN_1680754 0.6265542 0.4350649

Total number of rows: 47311

Table truncated, full table size 1403 Kbytes.




Supplementary file Size Download File type/resource
GSM1841866_9020374065_A_Grn.idat.gz 1.6 Mb (ftp)(http) IDAT
Processed data included within Sample table

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