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Sample GSM1841868 Query DataSets for GSM1841868
Status Public on Feb 09, 2017
Title SKHep1 rep3
Sample type RNA
 
Source name SKHep1 cell line
Organism Homo sapiens
Characteristics gender: male
sample type: cell line
disease state: cancer
cell line: SKHep1 cell line
Growth protocol HepG2, SKHep1 and MCF-7 cells were maintained in MEM medium (Gibco, Invitrogen, Life Technologies). MCF7 cells were cultured with 0.01 mg/ml of insulin (Invitrogen, Carlsbad, California). MDA-MB-231 cells were cultured in Dulbecco’s modified Eagle’s medium (Invitrogen). PC3 and LNCaP were cultured in RPMI1640 media (Gibco, Invitrogen, Life Technologies). All media were supplemented with 2 mmol/L glutamine (Sigma-Aldrich), 10% FBS (Gibco), 1 U/mL penicillin and 1 mg/mL streptomycin (Gibco).
Extracted molecule total RNA
Extraction protocol RNA was
Label biotin
Label protocol biotin-labeled cRNA using Illumina® TotalPrep RNA Amplification Kit, according to manufacturer’s instructions (Life Technologies)
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 3
Data processing The data were normalised using quantile normalisation with Genome Studio expression module
 
Submission date Jul 31, 2015
Last update date Feb 09, 2017
Contact name David Cheishvili
E-mail(s) david.cheishvili@mcgill.ca
Phone 5143987107
Organization name McGill
Street address ​3655 Sir William Osler
City Montreal
State/province Quebec
ZIP/Postal code H3G 1Y6
Country Canada
 
Platform ID GPL10558
Series (2)
GSE71625 A common promoter hypomethylation signature in invasive breast, liver and prostate cancer cell lines reveals novel targets involved in cancer invasiveness (expression)
GSE71627 A common promoter hypomethylation signature in invasive breast, liver and prostate cancer cell lines reveals novel targets involved in cancer invasiveness

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 -5.70318 0.725974
ILMN_2055271 27.34801 0.005194805
ILMN_1736007 11.79315 0.08051948
ILMN_2383229 -4.27138 0.6493506
ILMN_1806310 6.386648 0.2012987
ILMN_1779670 -3.491176 0.6220779
ILMN_1653355 11.68586 0.08311688
ILMN_1717783 -13.16963 0.9532468
ILMN_1705025 -0.6354716 0.4779221
ILMN_1814316 -3.316162 0.6155844
ILMN_2359168 -10.21932 0.8974026
ILMN_1731507 -16.94508 0.9831169
ILMN_1787689 6.607797 0.2012987
ILMN_3241953 134.3354 0
ILMN_1745607 -19.22263 0.9948052
ILMN_2136495 -11.40577 0.9246753
ILMN_1668111 -2.931943 0.5896104
ILMN_2295559 -1.539672 0.5064935
ILMN_1735045 70.63722 0
ILMN_1680754 15.29175 0.04545455

Total number of rows: 47311

Table truncated, full table size 1393 Kbytes.




Supplementary file Size Download File type/resource
GSM1841868_8909358064_F_Grn.idat.gz 1.6 Mb (ftp)(http) IDAT
Processed data included within Sample table

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