|
| Status |
Public on Jul 10, 2017 |
| Title |
Day 8 effector P14 total RNA replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Day 8 effector P14 total RNA
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
host strain background: B6
tissue: Spleen
cell type: effector P14 CD8 T cells at day 8 after LCMV infection (peak of CD8 T cell response)
molecule subtype: Total RNA
|
| Growth protocol |
P14 transgenic CD8 T cells specific for GP33 epitope of lymphocytic choriomeningitis virus (LCMV) were adoptively transferred into B6 mice, followed by LCMV Armstrong infection. Day 5 effector, day 8 effector, and memory (day 40~60 post infection) P14 CD8 T cells were purified from the LCMV-infected mice. Naive P14 CD8 T cells were directly purified from P14 transgenic mice. Total and polysome-associated (3 or more ribosomes) mRNAs were isolated from these purified P14 cells, and microarray analyses were performed.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA and polysome-associated RNA was performed according to the manufacturer's instructions. RNA was treated by DNase, followed by column-purification of RNA.
|
| Label |
biotin
|
| Label protocol |
RNA was ampified and labeled by Ovation Pico WTA System V2 (Nugen) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridization was performed at Dana-Farber Cancer Institute.
|
| Scan protocol |
Scan was performed at Dana-Farber Cancer Institute.
|
| Description |
D8_Total_2
|
| Data processing |
Data were preprocessed and normalized with robust multichip averaging in GenePattern (Broad Institute).
|
| |
|
| Submission date |
Aug 03, 2015 |
| Last update date |
Jul 10, 2017 |
| Contact name |
Koichi Araki |
| E-mail(s) |
karaki@emory.edu
|
| Organization name |
Emory University School of Medicine
|
| Department |
Department of Microbiology and Immunology
|
| Street address |
1510 Clifton Rd
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE71643 |
Translational profiling of in vivo virus-specific CD8 T cells |
|
