|
Status |
Public on Aug 10, 2015 |
Title |
cph2 deletion mutant rep 1 |
Sample type |
SRA |
|
|
Source name |
fungus
|
Organism |
Candida albicans |
Characteristics |
genotype/variation: cph2 deletion mutant
|
Treatment protocol |
mRNA was enriched using the Invitrogen Dynabeads mRNA purification kit
|
Growth protocol |
Wild type and cph2 mutant cells were grown in YPD overnight to OD600 >10
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Total RNA was extracted using the RiboPure kit from Amgion along with Dnase digestion as in the manual Library was constructed using the NuGen Encore Complete RNA-Seq Library Systems kit RNA-Seq was carried out using the Illumina HiSeq2500
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
Processing & Demultiplexing: Illumina Casava 1.7 Alignments: Illumina Eland v2e with default parameters Gene Expression Results / RPKM values: Illumina Casava VDC 1.7 on the reference genome Ca21 Differential Analysis: Cyber-T Genome_build: C. albicans SC5314 A21 (Ca21) Supplementary_files_format_and_content: tab-delimited text file that contain differential analysis of WT vs Cph2 deletion
|
|
|
Submission date |
Aug 10, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Haoping Liu |
E-mail(s) |
h4liu@uci.edu
|
Phone |
949-824-1137
|
Organization name |
University of California
|
Department |
Dept. of Biological Chemistry
|
Street address |
Med. Sci. I, Rm D234
|
City |
Irvine |
State/province |
CA |
ZIP/Postal code |
926197 |
Country |
USA |
|
|
Platform ID |
GPL19036 |
Series (2) |
GSE71902 |
Function and regulation of Cph2 in Candida albicans [RNA-Seq] |
GSE71904 |
Function and regulation of Cph2 in Candida albicans |
|
Relations |
SRA |
SRX1140555 |
BioSample |
SAMN03979721 |