|
| Status |
Public on Aug 10, 2015 |
| Title |
cph2 deletion mutant rep 2 |
| Sample type |
SRA |
| |
|
| Source name |
fungus
|
| Organism |
Candida albicans |
| Characteristics |
genotype/variation: cph2 deletion mutant
|
| Treatment protocol |
mRNA was enriched using the Invitrogen Dynabeads mRNA purification kit
|
| Growth protocol |
Wild type and cph2 mutant cells were grown in YPD overnight to OD600 >10
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted using the RiboPure kit from Amgion along with Dnase digestion as in the manual
Library was constructed using the NuGen Encore Complete RNA-Seq Library Systems kit
RNA-Seq was carried out using the Illumina HiSeq2500
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Processing & Demultiplexing: Illumina Casava 1.7
Alignments: Illumina Eland v2e with default parameters
Gene Expression Results / RPKM values: Illumina Casava VDC 1.7 on the reference genome Ca21
Differential Analysis: Cyber-T
Genome_build: C. albicans SC5314 A21 (Ca21)
Supplementary_files_format_and_content: tab-delimited text file that contain differential analysis of WT vs Cph2 deletion
|
| |
|
| Submission date |
Aug 10, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Haoping Liu |
| E-mail(s) |
h4liu@uci.edu
|
| Phone |
949-824-1137
|
| Organization name |
University of California
|
| Department |
Dept. of Biological Chemistry
|
| Street address |
Med. Sci. I, Rm D234
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
926197 |
| Country |
USA |
| |
|
| Platform ID |
GPL19036 |
| Series (2) |
| GSE71902 |
Function and regulation of Cph2 in Candida albicans [RNA-Seq] |
| GSE71904 |
Function and regulation of Cph2 in Candida albicans |
|
| Relations |
| SRA |
SRX1140556 |
| BioSample |
SAMN03980242 |
