|
| Status |
Public on Aug 11, 2015 |
| Title |
PDVC57 |
| Sample type |
RNA |
| |
|
| Source name |
Squamous skin cancer cell line
|
| Organism |
Mus musculus |
| Characteristics |
cell line: PDVC57
phenotype: malignant squamous
|
| Growth protocol |
Cells were cultured in DMEM supplemented with 10% foetal bovine serum (FBS), 1% penicillin-streptomycin and 4 mM L-glutamine at 37ºC and 5% of CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were isolated using the Qiagen™ kit (miRNA easy) following the manufacturer’s instructions
|
| Label |
Cy3
|
| Label protocol |
1µg of total RNA was labelled with the Exiqon Kit protocol following the manufacturer´s instructions.
|
| |
|
| Hybridization protocol |
Samples were heated at 95º C in darkness, hybridized, and washed with the robotic HS 4800 Pro system (Tecan®).
|
| Scan protocol |
Fluorescence was scanned with a GenePix 4000B (Axon Instruments™) microarray scanner.
Raw data processing was performed with the ExiMiR package from R
|
| Description |
origin: PDVC57 was established from a tumor resulting from injection of PDV cells into C57BI mice. PDV cells were derived by in vitro transformation with DMBA of primary keratinocytes from C57B1 mice
Quintanilla M, et al.,. Carcinogen-specific mutation and amplification of Ha-ras during mouse skin carcinogenesis. Nature. 1986 Jul 3-9;322(6074):78-80. PubMed PMID: 3014349.
|
| Data processing |
Image processing was accomplished with the Gene Prix Pro (v 6.0), and data processing was performed with the ExiMiR package for R (Gubian S, 2012; RDevelopment-Core-Team, 2010).
|
| |
|
| Submission date |
Aug 10, 2015 |
| Last update date |
Jun 17, 2016 |
| Contact name |
Jesús Pérez Losada |
| E-mail(s) |
jperezlosada@usal.es
|
| Organization name |
Inst. de Inv. Biomédica de Salamanca (IBSAL). H. U. de Salamanca. Inst. de Biología Molecular y Celular del Cáncer (IBMCC).
|
| Department |
Centro de inv. del Cáncer (CIC).
|
| Street address |
Campus Miguel de Unamuno
|
| City |
Salamanca |
| ZIP/Postal code |
E37007 |
| Country |
Spain |
| |
|
| Platform ID |
GPL20794 |
| Series (1) |
| GSE71923 |
miR-203 and miR-205 expression patterns identify subgroups of prognosis in cutaneous squamous cell carcinoma |
|
