|
| Status |
Public on Sep 30, 2015 |
| Title |
N2-OP50-prep30 |
| Sample type |
RNA |
| |
|
| Source name |
N2 exposed to OP50 for 4 hours
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
genotype: N2
bacteria: OP50
|
| Treatment protocol |
Animals were transferred to SK plates seeded with OP50 or PA14 4 hours prior to harvesting.
|
| Growth protocol |
Embryos were collected by bleaching and allowed to hatch and then arrest in the absence of food. Arrested L1 larvae were placed onto NGM plates seeded with OP50 and grown at 20oC to the young adult stage. Worms were washed from these plates and transferred to SK plates seeded with OP50 or PA14. After 4 hours, animals were harvested for analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRI reagent (Molecular Research Center)
|
| Label |
biotin
|
| Label protocol |
Affymetrix
|
| |
|
| Hybridization protocol |
Affymetrix
|
| Scan protocol |
Affymetrix
|
| Description |
wild-type worms, no infection
|
| Data processing |
GenePattern (Broad Institute)
|
| |
|
| Submission date |
Aug 13, 2015 |
| Last update date |
Sep 30, 2015 |
| Contact name |
Jennifer R Powell |
| E-mail(s) |
jpowell@gettysburg.edu
|
| Organization name |
Gettysburg College
|
| Department |
Biology
|
| Street address |
300 N. Washington Street
|
| City |
Gettysburg |
| State/province |
PA |
| ZIP/Postal code |
17325 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE72029 |
C. elegans gene expression in healthy and PA14-infected wild-type and fshr-1 mutant worms |
|
