|
| Status |
Public on Aug 26, 2015 |
| Title |
AC_lacZ injected_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
animal caps injected with lacZ mRNA
|
| Organism |
Xenopus laevis |
| Characteristics |
tissue: animal caps
treatment: injected with lacZ mRNA
|
| Treatment protocol |
Xenopus embryos were injected with mRNAs in the animal pole at two-cell stage. The animal caps were dissected at stage 9 using forceps in 1×MMR on 1% agar plate.
|
| Growth protocol |
Xenopus embryos were incubated in 0.2×MMR at 16°C and staged according to Nieuwkoop and Faber. The dissected animal caps were cultured in L-15 medium (67% L-15, 7.5 mM Tris-HCl pH7.5, 1 mg/ml BSA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the TRIzol reagent according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated aRNA was synthesized through in vitro transcription according to the standard Affymetrix protocol (GeneChip® 3' IVT Express Kit user manual).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 μg of aRNA were hybridized for 16 hr at 45°C on GeneChip® Xenopus laevis 2.0 Genome Array (64 Format). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from lacZ injected animal caps
9_lacZ
|
| Data processing |
The data were analyzed with Partek Genomics Suite with default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Aug 26, 2015 |
| Last update date |
Aug 26, 2015 |
| Contact name |
Igor Dawid |
| E-mail(s) |
idawid@nih.gov
|
| Organization name |
NICHD, NIH
|
| Street address |
6 Center Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL10756 |
| Series (1) |
| GSE72391 |
Identification of Kctd15 target genes in Xenopus animal caps injected with wnt3a and chordin |
|
