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Status |
Public on Aug 26, 2015 |
Title |
AC_kctd15 injected_rep3 |
Sample type |
RNA |
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Source name |
animal caps injected with kctd15 mRNA
|
Organism |
Xenopus laevis |
Characteristics |
tissue: animal caps treatment: injected with kctd15 mRNA
|
Treatment protocol |
Xenopus embryos were injected with mRNAs in the animal pole at two-cell stage. The animal caps were dissected at stage 9 using forceps in 1×MMR on 1% agar plate.
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Growth protocol |
Xenopus embryos were incubated in 0.2×MMR at 16°C and staged according to Nieuwkoop and Faber. The dissected animal caps were cultured in L-15 medium (67% L-15, 7.5 mM Tris-HCl pH7.5, 1 mg/ml BSA).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the TRIzol reagent according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated aRNA was synthesized through in vitro transcription according to the standard Affymetrix protocol (GeneChip® 3' IVT Express Kit user manual).
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|
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Hybridization protocol |
Following fragmentation, 10 μg of aRNA were hybridized for 16 hr at 45°C on GeneChip® Xenopus laevis 2.0 Genome Array (64 Format). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
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Description |
Gene expression data from animal caps injected with kctd15 mRNA 10_kctd15
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Data processing |
The data were analyzed with Partek Genomics Suite with default analysis settings and global scaling as normalization method.
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|
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Submission date |
Aug 26, 2015 |
Last update date |
Aug 26, 2015 |
Contact name |
Igor Dawid |
E-mail(s) |
idawid@nih.gov
|
Organization name |
NICHD, NIH
|
Street address |
6 Center Drive
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL10756 |
Series (1) |
GSE72391 |
Identification of Kctd15 target genes in Xenopus animal caps injected with wnt3a and chordin |
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