|
Status |
Public on Jun 30, 2016 |
Title |
Unaffected Relative 3 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
blood leukocytes, unaffected
|
Organism |
Homo sapiens |
Characteristics |
disease status: unaffected cell type: blood leukocytes gender: male
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was isolated using the FlexiGene DNA kit as per manufacturer's recommendations.
|
Label |
Cy5
|
Label protocol |
As per manufacturer's instructions. Protocol: Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis - Enzymatic Labeling for Blood Cells or Tissues - Version 6,3, October 2010.
|
|
|
Channel 2 |
Source name |
Human reference DNA (Agilent 5190-4371)
|
Organism |
Homo sapiens |
Characteristics |
gender: female sample type: reference
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was isolated using the FlexiGene DNA kit as per manufacturer's recommendations.
|
Label |
Cy3
|
Label protocol |
As per manufacturer's instructions. Protocol: Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis - Enzymatic Labeling for Blood Cells or Tissues - Version 6,3, October 2010.
|
|
|
|
Hybridization protocol |
As per manufacturer's instructions. Protocol: Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis - Enzymatic Labeling for Blood Cells or Tissues - Version 6,3, October 2010.
|
Scan protocol |
Agilent Feature Extraction V 11.5.1.
|
Description |
Unaffected 3
|
Data processing |
Agilent CytoGenomics 3.0.1.1 software. Genome build hg19. Default Analysis Method - CGH+SNP v2. Normalization: GC correction and Diploid Peak Centralization.
|
|
|
Submission date |
Sep 01, 2015 |
Last update date |
Jun 30, 2016 |
Contact name |
Anthony J. Aldave |
E-mail(s) |
Aldave@jsei.ucla.edu
|
Organization name |
Stein Eye Institute, UCLA
|
Department |
Ophthalmology
|
Lab |
Cornea Genetics Laboratory
|
Street address |
200 Stein Plaza DSERC 3-143
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90095 |
Country |
USA |
|
|
Platform ID |
GPL20879 |
Series (1) |
GSE72617 |
Identification of Potentially Pathogenic Variants in the Posterior Polymorphous Corneal Dystrophy 1 Locus |
|