|
| Status |
Public on Mar 29, 2016 |
| Title |
Cell line [HeLa Hyp] |
| Sample type |
RNA |
| |
|
| Source name |
Uterine cervical cancer
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa
histology: Squamous cell carcinoma
exposed to: hypoxic (0.2% O2, 5% CO2) conditions for 24 h at 37°C
|
| Treatment protocol |
Cells were plated out in plastic dishes (3x10^5 cells for 6 cm dishes, 9x10^5 for 10 cm dishes) 24 h prior to exposure to hypoxic (0.2% O2, 5% CO2) or normoxic (95% air, 5% Co2) conditions for 24 h at 37°C. The hypoxia treatment was performed in an Invivo2200 chamber (Ruskinn Technology Ltd, Bridgend, UK) with accurate O2 and CO2 controls.
|
| Growth protocol |
The cells were incubated in Dulbecco's Modified Eagle medium with GlutaMAX supplemented with 10% fetal calf serum (FCS) and 100 U/ml penicillin/streptomycin (Gibco) under a 5% CO2 atmosphere at 37°C, and subcultured twice a week.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy MiniKit (Quiagen), according to the manufacturers’ instructions. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
HeLa Hyp.AVG_Signal
|
| Data processing |
The data were normalised using quantile normalisation in BeadStudio 3.1.3.0
|
| |
|
| Submission date |
Sep 04, 2015 |
| Last update date |
Mar 29, 2016 |
| Contact name |
Heidi Lyng |
| E-mail(s) |
heidi.lyng@rr-research.no
|
| Phone |
4722781478
|
| Organization name |
Oslo University Hospital
|
| Department |
Department of Radiation Biology
|
| Street address |
Montebello
|
| City |
Oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE72723 |
Integrative analysis of DCE-MRI and gene expression profiles in construction of a gene classifier for assessment of hypoxia-related risk of chemoradiotherapy failure in cervical cancer |
| GSE75034 |
Selection of reference genes for gene expression studies related to hypoxia in cervical cancer |
|
