|
| Status |
Public on Oct 24, 2016 |
| Title |
Col h-3R |
| Sample type |
SRA |
| |
|
| Source name |
Leaves
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: leaves
ecotype: Col-0
age: Twenty-five-day-old plants
treatment: Treated with 44oC heat stress for 2h
|
| Treatment protocol |
All treatments were perfomed in parallel. Salt stress was imposed on 12-d-old plants by applying 150mM NaCl for 13 days. Twenty five-d-old plants grown in the presense or absense of salt stress were subjected to heat stress (44oC) for 1h.
|
| Growth protocol |
Plants were grown on soil mixture under controlled conditions: 21oC, 12-h light cycle, 100 mmol m-2s-1, and relative humidity of 70%
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by using Trizol reagent and purified using Qiagen RNeasy mini kit
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Used Tophat and Bowtie for read alignment onto Arabidopsis reference genome and Cufflinks for obtaining the FPKMs and assessment of significance of fold change
Genome_build: TAIR9
Supplementary_files_format_and_content: Tab-delimited file (opens in Excel); FPKMs for Control, Salt, Heat, and Salt-Heat combination stress
|
| |
|
| Submission date |
Sep 08, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Rajeev K. Azad |
| Organization name |
University of North Texas
|
| Street address |
1155 Union Circle #305220
|
| City |
Denton |
| State/province |
Texas |
| ZIP/Postal code |
76210 |
| Country |
USA |
| |
|
| Platform ID |
GPL13222 |
| Series (1) |
| GSE72806 |
ABA is required for plant acclimation to a combination of salt and heat stress |
|
| Relations |
| BioSample |
SAMN04040988 |
| SRA |
SRX1206679 |
