|
| Status |
Public on Aug 15, 2007 |
| Title |
CF Sputum in vivo replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
CF Sputum in vivo replicate2
|
| Organism |
Pseudomonas aeruginosa |
| Characteristics |
Pseudomonas aeruginosa total RNA was isolated directly from CF sputum patient 42
|
| Biomaterial provider |
Dr. Wallace J. Matthews
|
| Treatment protocol |
Sputum samples were maintained on ice at all times. Sputum was treated with Sputolysin, proteinase K, DNase I, and pelleted. Cell pellet was washed twice with COLD double distilled water. Cell pellet was then resuspended in TRIzol reagent and sonicated for 10 secs at maximum level three times. All genetic material was then phenol:chloroform extracted and ethanol precipitated, resuspended in DEPC-treated water and processed as per S. Lory protocol.
|
| Growth protocol |
1x M9 minimal media supplemented with 20 mM citrate
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol treated Pseudomonas aeruginosa total RNA was isolated according to protocol developed by S. Lory, and cleanded up with QIAgen RNeasy with extra oncolumn DNase I treatment
|
| Label |
Biotin
|
| Label protocol |
As per Affymetrix protocol for terminal labeling (used EnzoArray)
|
| |
|
| Hybridization protocol |
As per Affymetrix protocol
|
| Scan protocol |
As per Affymetrix protocol
|
| Description |
Samples processed at Greenwood Biotechnology Facility (Honolulu, HI)
|
| Data processing |
Signal Values determined using MAS5
|
| |
|
| Submission date |
May 03, 2007 |
| Last update date |
Jun 21, 2007 |
| Contact name |
Tung Hoang |
| E-mail(s) |
yunkang@hawaii.edu
|
| Phone |
808-956-5035
|
| Organization name |
University of Hawaii at Manoa
|
| Department |
Microbiology
|
| Lab |
LSB 318
|
| Street address |
1800 East-West Road
|
| City |
Honolulu |
| State/province |
HI |
| ZIP/Postal code |
96822 |
| Country |
USA |
| |
|
| Platform ID |
GPL84 |
| Series (1) |
| GSE7704 |
In vivo evidence of Pseudomonas aeruignosa nutrient acquisition and pathogenesis in the cystic fibrosis lung |
|
