|
| Status |
Public on Mar 31, 2023 |
| Title |
C14_0452_2 |
| Sample type |
RNA |
| |
|
| Source name |
B6-Chr14MSM_mouse liver
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: B6-Chr14MSM
tissue: liver
age: 10-week-old
|
| Treatment protocol |
Samples were collected after a fasting period of four hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Livers were immersed in RNAlater (life technologies) and stored at -20C. Total RNA was extracted by RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 10ug total RNA by using GeneChip 3'IVT express kit (Affymetrix) according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Genechip scanner 3000 (Affymetrix).
|
| Description |
Gene expression data from liver at 10-week-old male
|
| Data processing |
The data were analyzed with DFW (Chen et al. Bioinformatics, 2007).
|
| |
|
| Submission date |
Sep 09, 2015 |
| Last update date |
Mar 31, 2023 |
| Contact name |
Toyoyuki Takada |
| E-mail(s) |
ttakada@nig.ac.jp
|
| Organization name |
National Institute of Genetics
|
| Lab |
Mammalian Genetics Laboratory
|
| Street address |
Yata-1111
|
| City |
Mishima |
| State/province |
Shizuoka-ken |
| ZIP/Postal code |
411-8540, |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE72825 |
Expression data from liver of male mouse at 10-week-old |
|
