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Status |
Public on Mar 31, 2023 |
Title |
C15_0845_2 |
Sample type |
RNA |
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Source name |
B6-Chr15MSM_mouse liver
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Organism |
Mus musculus |
Characteristics |
gender: male strain: B6-Chr15MSM tissue: liver age: 10-week-old
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Treatment protocol |
Samples were collected after a fasting period of four hours.
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Extracted molecule |
total RNA |
Extraction protocol |
Livers were immersed in RNAlater (life technologies) and stored at -20C. Total RNA was extracted by RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared from 10ug total RNA by using GeneChip 3'IVT express kit (Affymetrix) according to the standard Affymetrix protocol.
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Hybridization protocol |
Following fragmentation, 12.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Genechip scanner 3000 (Affymetrix).
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Description |
Gene expression data from liver at 10-week-old male
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Data processing |
The data were analyzed with DFW (Chen et al. Bioinformatics, 2007).
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Submission date |
Sep 09, 2015 |
Last update date |
Mar 31, 2023 |
Contact name |
Toyoyuki Takada |
E-mail(s) |
ttakada@nig.ac.jp
|
Organization name |
National Institute of Genetics
|
Lab |
Mammalian Genetics Laboratory
|
Street address |
Yata-1111
|
City |
Mishima |
State/province |
Shizuoka-ken |
ZIP/Postal code |
411-8540, |
Country |
Japan |
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|
Platform ID |
GPL1261 |
Series (1) |
GSE72825 |
Expression data from liver of male mouse at 10-week-old |
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