|
| Status |
Public on Sep 15, 2015 |
| Title |
1450-750K |
| Sample type |
genomic |
| |
|
| Source name |
myelodysplastic syndromes
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: myelodysplastic syndromes
cell type: primary MDS mononuclear cells
|
| Treatment protocol |
None
|
| Growth protocol |
primary MDS mononuclear cells, no growth protocol
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from mononuclear cell preparations of primary MDS mononuclear cells at diagnosis using the Qiagen DNA Blood Mini Kit. DNA quality and quantity was assessed using a Spectrophotometer and agarose gel electrophoresis.
|
| Label |
Biotin
|
| Label protocol |
Labelling and hybridizations were performed according to protocols from Affymetrix
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to the array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner7G.
|
| Description |
Hybridized to 750K
|
| Data processing |
The data were analyzed using the Nexus Copy Number (version 7.5, Biodiscovery Inc., El Segundo, CA,USA) software program.Data was normalized using the SNP-FASST2 segmentation algorithm.
Normalized probe intensity and allele ratio data were visualized in Nexus v7.5.
|
| |
|
| Submission date |
Sep 14, 2015 |
| Last update date |
Sep 15, 2015 |
| Contact name |
Feng Xu |
| E-mail(s) |
xvfeng3619@163.com
|
| Organization name |
Shanghai Jiaotong University Affiliated Sixth People's Hospital
|
| Department |
Hematology
|
| Street address |
600 Yishan Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200233 |
| Country |
China |
| |
|
| Platform ID |
GPL18637 |
| Series (1) |
| GSE73005 |
Affymetrix SNP array data for myelodysplastic syndromes (MDS) samples |
|
