|
| Status |
Public on Sep 18, 2015 |
| Title |
metastatic tumor (lung nodule No.3) |
| Sample type |
RNA |
| |
|
| Source name |
metastatic lung nodule from rat glioma (C6-L cell) xenograft model
|
| Organism |
Rattus norvegicus |
| Characteristics |
host strain: BALB/c nu/nu nude mice
age: 6 weeks
tissue: metastatic lung nodule
time point: 6 weeks after ɣ-irradiation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from tissue or cell using RNAeasy Mini Kit (Qiagen, Valencia, CA, USA)
|
| Label |
Cy3
|
| Label protocol |
Labeled cRNA were prepared from 1~5 ug total RNA using the Agilent’s Quick Amp Labeling Kit
|
| |
|
| Hybridization protocol |
Following fragmentation, 1.65 ug of cRNA were hybridized to the Agilent expression microarray according to the protocols provided by the manufacturer
|
| Scan protocol |
Arrays were scanned using the Agilent Technologies G2600D SG12494263
|
| Description |
N_Metastatic Tumor 3
|
| Data processing |
Quantile normalization: Microarray results were extracted using Agilent Feature Extraction software v11.0 (Agilent Technologies) The raw data for same gene was then summarized automatically in Agilent feature extraction protocol to generate raw data text file, providing expression data for each gene probed on the array. Array probes that have Flag A in samples were filtered out. The selected gProcessedSignal value was transformed by logarithm and normalized by quantile method, resulting in 18,502 data rows.
|
| |
|
| Submission date |
Sep 17, 2015 |
| Last update date |
Sep 18, 2015 |
| Contact name |
SuJin Jang |
| E-mail(s) |
akentnwls@naver.com
|
| Organization name |
KIRAMS
|
| Street address |
215-4, Gongneung-dong, Nowon-gu
|
| City |
Seoul |
| ZIP/Postal code |
139-706 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL15084 |
| Series (1) |
| GSE73116 |
Detecting metastatic tumors after γ-irradiation using longitudinal molecular imaging and gene expression profiling of metastatic tumor nodules |
|
