|
| Status |
Public on Oct 17, 2017 |
| Title |
PKC 12hr III |
| Sample type |
RNA |
| |
|
| Source name |
Primary adult human keratinocytes
|
| Organism |
Homo sapiens |
| Characteristics |
type: Primary cell (Human Keratinocyte)
treatment: PKC for 12hrs
tissue: epidermis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
The data were normalised using quantile normalisation with MatLab
|
| |
|
| Submission date |
Sep 17, 2015 |
| Last update date |
Oct 17, 2017 |
| Contact name |
ajay Mishra |
| E-mail(s) |
ajay.mishra@kcl.ac.uk
|
| Organization name |
King's College London
|
| Department |
Centre for Stem Cells and Regenerative Medicine
|
| Street address |
Great Maze Pond
|
| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10904 |
| Series (1) |
| GSE73147 |
A protein phosphatase network controls the temporal and spatial dynamics of differentiation commitment in human epidermis |
|
