|
| Status |
Public on Dec 24, 2015 |
| Title |
neonatal rat skps treated with alprostadil, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
neonatal rat skps treated with alprostadil
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
treatment group: Alprostadil
|
| Treatment protocol |
Secondary SKPs spheres were dissociated, treated with 100 nM of alprostadil, trimebutine maleate or 100 nM of both trimebutine maleate and trametinib for 24 hour
|
| Growth protocol |
Cells were cultured at 50,000 cells/ml in SKPs basal growth medium (DMEM-F12, 3:1 and 40 ng/ml FGF2, 20 ng/ml EGF, 2% B27, and 1 μg/ml fungizone) and allowed to form spheres
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Ambion Ribopure kit as described by the manufacturer
|
| Label |
biotin
|
| Label protocol |
cDNA was prepared using the Ambion WT Expression Kit using 400 ng of total RNA
|
| |
|
| Hybridization protocol |
Affymetrix Rat Gene 2.0 ST arrays were washed, stained and scanned according to the Fluidics Protocol FS450_0002 using 5.5 ug of cDNA
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression of neonatal rat SKPs treated with alprostadil for 24 hours.
|
| Data processing |
The data were analyzed using the oligo package of Bioconductor in R. The data were background corrected, normalized with quantile normalization, transformed into the log2 scale, and summarized into probesets using the Robust Multichip Analysis (RMA) method.
|
| |
|
| Submission date |
Sep 22, 2015 |
| Last update date |
Dec 24, 2015 |
| Contact name |
Freda Miller |
| E-mail(s) |
fredam@sickkids.ca
|
| Organization name |
Hospital for Sick Children
|
| Street address |
686 Bay St
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 0A4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL17117 |
| Series (2) |
| GSE73328 |
Identification of drugs that enhance skin repair using dermal stem cell-based screens [Rat] |
| GSE73329 |
Identification of drugs that enhance skin repair using dermal stem cell-based screens |
|
