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Sample GSM1891412 Query DataSets for GSM1891412
Status Public on Apr 22, 2016
Title iEP-derived red colonies, biological rep1
Sample type RNA
 
Source name Mouse colonies derived from day 5-iEPs
Organism Mus musculus
Characteristics assay: Colony assay
Treatment protocol Cells were pelleted at 350g and resuspended in 350ul of RLT buffer (Qiagen) and frozen at -80°C.
Growth protocol For colony-forming assays MethoCult M3236 (Stem Cell Technologies) was used and supplemented with 5U/mL rhEPO, 50ng/mL mSCF, 100nM dexamethasone and 1% antibiotics. Cells were resuspended in FCS and added to MethoCult mixture. Colonies were scored at day 8.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with RNAeasy micro kit (Qiagen) according to the manufacturers protocol.
Label biotin
Label protocol Affymetrix protocol
 
Hybridization protocol Samples were hybridised to the Affymetrix MoGene 2.1 array accroding to Affymetrix protocols
Scan protocol Scanning was performed according to Affymetrix protocols
Description Gene expression data from visibly red colonies derived from day 5-iEPs
iEP_red1
Data processing Signal intensities from the MAS5 suite were RMA summarised
 
Submission date Sep 23, 2015
Last update date Apr 23, 2016
Contact name Shamit Soneji
Organization name BMC
Street address Solvegatan 19
City Lund
ZIP/Postal code 22184
Country Sweden
 
Platform ID GPL17400
Series (1)
GSE73344 Expression data from mouse induced Erythroid Progenitor (iEP)-derived colonies, mouse bone marrow and fetal liver BFU-Es and mouse fibroblasts.

Data table header descriptions
ID_REF
VALUE RMA normalised signal intensity

Data table
ID_REF VALUE
17200001 8.478
17200003 43.599
17200005 7.558
17200007 3.202
17200009 2.144
17200011 2.675
17200013 2.094
17200015 1.918
17200017 7.768
17200019 1.883
17200021 2.284
17200023 21.189
17200025 3.663
17200027 18.017
17200029 1.62
17200031 4.232
17200033 3.233
17200035 1.254
17200037 4.42
17200039 3.382

Total number of rows: 41345

Table truncated, full table size 619 Kbytes.




Supplementary file Size Download File type/resource
GSM1891412_A941_04_iEP_RBC_1.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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