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Sample GSM1891422 Query DataSets for GSM1891422
Status Public on Apr 22, 2016
Title adult bone marrow BFU-Es, rep1
Sample type RNA
 
Source name Mouse BFU-E colonies derived from bone marrow cells
Organism Mus musculus
Characteristics assay: Colony assay
Treatment protocol Cells were pelleted at 350g and resuspended in 350ul of RLT buffer (Qiagen) and frozen at -80°C.
Growth protocol For colony-forming assays MethoCult M3236 (Stem Cell Technologies) was used and supplemented with 5U/mL rhEPO, 50ng/mL mSCF, 100nM dexamethasone and 1% antibiotics. Cells were resuspended in FCS and added to MethoCult mixture. Colonies were scored at day 8.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with RNAeasy micro kit (Qiagen) according to the manufacturers protocol.
Label biotin
Label protocol Affymetrix protocol
 
Hybridization protocol Samples were hybridised to the Affymetrix MoGene 2.1 array accroding to Affymetrix protocols
Scan protocol Scanning was performed according to Affymetrix protocols
Description Gene expression data from BFU-E colonies derived from total mouse bone marrow cells
BM BFU-Es 1
Data processing Signal intensities from the MAS5 suite were RMA summarised
 
Submission date Sep 23, 2015
Last update date Apr 23, 2016
Contact name Shamit Soneji
Organization name BMC
Street address Solvegatan 19
City Lund
ZIP/Postal code 22184
Country Sweden
 
Platform ID GPL17400
Series (1)
GSE73344 Expression data from mouse induced Erythroid Progenitor (iEP)-derived colonies, mouse bone marrow and fetal liver BFU-Es and mouse fibroblasts.

Data table header descriptions
ID_REF
VALUE RMA normalised signal intensity

Data table
ID_REF VALUE
17200001 4.785
17200003 16.067
17200005 4.371
17200007 5.162
17200009 4.013
17200011 2.246
17200013 10.171
17200015 7.822
17200017 7.557
17200019 3.33
17200021 2.034
17200023 11.41
17200025 5.267
17200027 23.91
17200029 2.499
17200031 10.06
17200033 1.922
17200035 1.649
17200037 2.295
17200039 2.103

Total number of rows: 41345

Table truncated, full table size 620 Kbytes.




Supplementary file Size Download File type/resource
GSM1891422_A941_14_BM_colony_1.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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