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Sample GSM1891423 Query DataSets for GSM1891423
Status Public on Apr 22, 2016
Title adult bone marrow BFU-Es, rep2
Sample type RNA
 
Source name Mouse BFU-E colonies derived from bone marrow cells
Organism Mus musculus
Characteristics assay: Colony assay
Treatment protocol Cells were pelleted at 350g and resuspended in 350ul of RLT buffer (Qiagen) and frozen at -80°C.
Growth protocol For colony-forming assays MethoCult M3236 (Stem Cell Technologies) was used and supplemented with 5U/mL rhEPO, 50ng/mL mSCF, 100nM dexamethasone and 1% antibiotics. Cells were resuspended in FCS and added to MethoCult mixture. Colonies were scored at day 8.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with RNAeasy micro kit (Qiagen) according to the manufacturers protocol.
Label biotin
Label protocol Affymetrix protocol
 
Hybridization protocol Samples were hybridised to the Affymetrix MoGene 2.1 array accroding to Affymetrix protocols
Scan protocol Scanning was performed according to Affymetrix protocols
Description Gene expression data from BFU-E colonies derived from total mouse bone marrow cells
BM BFU-Es 2
Data processing Signal intensities from the MAS5 suite were RMA summarised
 
Submission date Sep 23, 2015
Last update date Apr 23, 2016
Contact name Shamit Soneji
Organization name BMC
Street address Solvegatan 19
City Lund
ZIP/Postal code 22184
Country Sweden
 
Platform ID GPL17400
Series (1)
GSE73344 Expression data from mouse induced Erythroid Progenitor (iEP)-derived colonies, mouse bone marrow and fetal liver BFU-Es and mouse fibroblasts.

Data table header descriptions
ID_REF
VALUE RMA normalised signal intensity

Data table
ID_REF VALUE
17200001 3.269
17200003 6.82
17200005 3.657
17200007 2.157
17200009 6.872
17200011 14.23
17200013 6.54
17200015 9.27
17200017 13.218
17200019 2.353
17200021 2.209
17200023 12.574
17200025 2.399
17200027 27.453
17200029 4.03
17200031 9.705
17200033 1.772
17200035 4.969
17200037 12.324
17200039 1.849

Total number of rows: 41345

Table truncated, full table size 620 Kbytes.




Supplementary file Size Download File type/resource
GSM1891423_A941_15_BM_colony_2.CEL.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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