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Sample GSM189342 Query DataSets for GSM189342
Status Public on Dec 31, 2007
Title heart_short-term heat acclimated rats (2dac)
Sample type RNA
 
Source name heart
Organism Rattus norvegicus
Characteristics Sabra strain, males, 3 mo old, heart
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from individual tissue samples using Tri-Reagent isolation protocol. Three pools (6 animals) were prepared for each experimental group.
Label [32P]dATP
Label protocol Standard Clontech protocol was used
 
Hybridization protocol The membranes were prehybridized for 1 h at 68°C in a hybridization solution (Clontech) containing 0.1 mg/ml sheared salmon testes DNA (Sigma) to block nonspecific binding. Synthesized radio labeled cDNA probe was applied to each membrane and hybridized overnight at 68°C. Cot-1 DNA (Clontech) (1 ug/ml) was added to block nonspecific binding.
Scan protocol For analysis, the membranes were exposed for 15 and 24 h to a phosphor screen and detected using a Bio-imaging Analyzer BAS2000 (Fuji Photo Film Co. Ltd Japan.
Description Gene expression data from left ventricle of short heat acclimated (34°C,2days)rat heart
Data processing We used Atlas Image 2.01 software (Clontech) to grid the phosphor image, to record the pixel density of each spot, and to perform background subtraction. The background-subtracted data were then further analyzed.
 
Submission date May 15, 2007
Last update date Jun 11, 2007
Contact name Michal Horowitz
E-mail(s) horowitz@cc.huji.ac.il
Phone 972 2 6757588
Fax 972 2 6439736
Organization name The hebrew University
Lab Environmental Physiology
Street address POB 12272
City Jerusalem
ZIP/Postal code 91120
Country Israel
 
Platform ID GPL153
Series (1)
GSE7799 Cardiac and Soleus muscles following exposure of rats to heat acclimation and exercise training

Data table header descriptions
ID_REF 1
VALUE Normalized signal; the experimental groups data are only those above the 1200 threshold point

Data table
ID_REF VALUE
R234 1.702
R246 0.486
RG076 0.987
RG146 3.309
R215 0.46
R438 0.688
RG007 1.753
RG299 0.485
R389 0.891
R071 0.743
R615 0.64
RG341 0.784
R428 0.66
R003 0.648
R196 0.64
R033 1.124
RG175 2.169
RG452 0.549
R115 0.587
R165 0.531

Total number of rows: 612

Table truncated, full table size 6 Kbytes.




Supplementary file Size Download File type/resource
GSM189342.txt.gz 6.9 Kb (ftp)(http) TXT
Processed data included within Sample table

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