|
| Status |
Public on May 03, 2016 |
| Title |
Homozygous AMPK R299Q Knock-in 4 |
| Sample type |
SRA |
| |
|
| Source name |
Hypothalamic Arcuate Nucleus
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: Homozygous AMPK R299Q
tissue: brain
|
| Growth protocol |
Ad Libitum Fed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The hypothalamic arcuate nucleus was extracted from 14um brain cryosections using laser capture microdissection. RNA was extracted usiing Qiagen RNeasy Plus Micro kit.
RNA was subjected to whole-transcriptome amplification using NuGEN RNAseq v2 System to make dsCDNA, this was followed by fragmentation to 200bp with a Diagenode Bioruptor Pico sonicator. The resulting fragments were then used to make bar-coded libraries using NuGEN Ovation Rapid DR Multiplex System
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
total RNA extracted from the hypothalamic arcuate nucleus from homozygous AMPK R299Q knock-in mice
Hom4
|
| Data processing |
Reads were aligned to the GRCm38/Ensembl V54 transcriptome using Tophat v2.0.8 with default parameters
Counts and differential gene expression were determined using CuffLinks v2.2.1 using default parameters
Genome_build: GRCm38
Supplementary_files_format_and_content: FPKM values in tab-delimited txt format
|
| |
|
| Submission date |
Sep 25, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Brian Yee Hong Lam |
| E-mail(s) |
yhbl2@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Metabolic Research Laboratories
|
| Street address |
Box 289 Addenbookes Hospital, Hills Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0QQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE73436 |
Chronic Activation of γ2 AMPK Induces Obesity and Reduces Beta Cell Function |
|
| Relations |
| BioSample |
SAMN04112617 |
| SRA |
SRX1287793 |
