|
| Status |
Public on Aug 30, 2007 |
| Title |
Donor 1 - control |
| Sample type |
RNA |
| |
|
| Source name |
Human elutriated monocytes
|
| Organism |
Homo sapiens |
| Characteristics |
Monocytes exposed to vehicle control (EtOH)
|
| Treatment protocol |
Monocytes were stimulated with LTD4 (100nM) or with vehicle (EtOH) control for 30 min. and total RNA was extracted.
|
| Growth protocol |
Monocytes cultured in RPMI1640, 10% heat inactivated fetal bovine serum, 2 mM L-glutamine overnight before stimulation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy mini kit with Dnase treatment (P/N 74104)
|
| Label |
biotin
|
| Label protocol |
Affymetix GeneChip Expression 3' amplification reagents for IVT labeling (P/N 900449) was used to prepare Biotinylated cRNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized (in the presence of DMSO) for 16 hr at 45C on Human Genome 133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetric Scanner 3000 6G model
|
| Description |
Elutriated monocytes from donor 1 treated with vehicle control for 30 min.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
May 15, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Grzegorz Woszczek |
| E-mail(s) |
gwoszczek@cc.nih.gov
|
| Organization name |
NIH
|
| Department |
Critical Care Medicine Department
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7807 |
Leukotriene D4 induces gene expression in human monocytes through cysteinyl leukotriene type I receptor. |
|
| Relations |
| Reanalyzed by |
GSE49910 |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE86362 |
| Reanalyzed by |
GSE119087 |
