|
| Status |
Public on Sep 25, 2007 |
| Title |
VT_Epidi_norm_26ans_corps |
| Sample type |
RNA |
| |
|
| Source name |
Normal epididimus, 26 years-old, corpus segment
|
| Organism |
Homo sapiens |
| Characteristics |
Seminal vesicles
|
| Growth protocol |
Tissues were collected under optimum conditions while artificial circulation was maintained to preserve organs assigned for transplantation. Epididymides were dissected in caput, corpus and cauda epididymidis and immediately frozen in liquid nitrogen and stored at -80°C until used for RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Trizol reagent according to manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared accordind to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentaion, 15 µg of cRNA were hybridized for 16h at 45°C to GeneChip U133 Plus 2.0 Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 (EukGE-WS2v5_450).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 G7 and extracted by GCOS 1.2 software (Affymetrix).
|
| Description |
biological rep 2
|
| Data processing |
The data were analyzed with LimmaGUI software (Bioconductor). Data was normalized by RMA method.
|
| |
|
| Submission date |
May 15, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ezequiel L Calvo |
| E-mail(s) |
cezequiel@yahoo.com
|
| Organization name |
CRCHUL
|
| Department |
Molecular Endocrinilogy
|
| Lab |
Microarrays
|
| Street address |
2705 Boul. Laurier
|
| City |
Quebec |
| State/province |
Quebec |
| ZIP/Postal code |
G1V 4G2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7808 |
Region specific gene expression profiling along the human epididymis |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
