|
| Status |
Public on Dec 09, 2015 |
| Title |
ESC-2 |
| Sample type |
SRA |
| |
|
| Source name |
Embryonic stem cells derived from mouse E3.5 embryo
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6 (B6)N
cell type: ESCs
|
| Treatment protocol |
Treatment protocol was described in Experimental Procedures section of the paper
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen).
RNA sequencing libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
The transcriptome reads were aligned to the mouse genome using TopHat program
Aligned reads were assembled using Cufflinks program.
The gene expression level was calculated by using FPKM method.
Genome_build: mm10
Supplementary_files_format_and_content: fpkm_tracking .txt file includes FPKM values for the analysis of gene expression
|
| |
|
| Submission date |
Sep 30, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Xu Zhang |
| E-mail(s) |
tiger314159@163.com
|
| Organization name |
Peking University
|
| Street address |
No.5 Yiheyuan Road
|
| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE73631 |
A XEN-like state bridges somatic cells to pluripotency during chemical reprogramming |
|
| Relations |
| BioSample |
SAMN04123942 |
| SRA |
SRX1297572 |
