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Status
Public on Mar 23, 2004
Title
267B1 vs. LNCaP. Array 4.
Sample type
genomic
Channel 1 Source name
prostate cell line 267B1 DNA, Cy3
Organism
Homo sapiens Extracted molecule
genomic DNA
Channel 2 Source name
prostate cell line LNCaP DNA, Cy5
Organism
Homo sapiens Extracted molecule
genomic DNA
Description
The methylation status of 267B1 and LNCaP are compared. DNA samples were digested with methylation sensitive enzyme HpaII, ligated with adaptor, PCR amplified and labeled with Cye Dye. The labeled target are hybridized with human promoter array. In general, methylated sequences will not be cut and will not be amplified. Only unmethylated sequences will be amplified and hybridized to arrays.
Submission date
Mar 20, 2004
Last update date
May 27, 2005
Contact name
Yipeng Wang
E-mail(s)
ywang@sdibr.org Phone
8585813960
Organization name
Vaccine Research Institute of San Diego
Street address
City
San Diego
State/province
CA
ZIP/Postal code
92121
Country
USA
Platform ID
GPL864 Series (1)
GSE951 267B1, PC3M, LNCaP Methylation Profile vs. mRNA Expression Level
Data table header descriptions ID_REF
CH1_RAW Intensity of spots. 267B1, Cy3.
CH1_BKD_RAW Intensity of spots background.
CH2_RAW Intensity of spots. PC3M, Cy5.
CH2_BKD_RAW Intensity of spots background.
VALUE Lowess normalized log base 2 ratio (Ch2/Ch1).
Data table ID_REF CH1_RAW CH1_BKD_RAW CH2_RAW CH2_BKD_RAW VALUE 1
358.170013
0
882.340027
15
-0.768046788
2
717.349976
0
814.969971
72.73333
0.263529905
3
463.600006
0
645.76001
90.066666
0.272409059
4
8112.419922
10.966666
6359.970215
59.266666
-0.257779534
5
920.960022
1.633333
1022.609985
50.633335
0.036901453
6
468.540009
4.366667
739.549988
27.233334
-0.159922559
7
1671.449951
0
1246.189941
0.433333
0.245981401
8
593.469971
0.3
722.719971
1
0.075597102
9
586.820007
0
844.140015
25.033333
-0.136497902
10
653.049988
0
1253.76001
0
-0.747528994
11
637.630005
0
997.960022
0
-0.347885536
12
891.039978
0
1471.640015
11.5
-0.723192953
13
1479.069946
0
1586.670044
12.233334
-0.270258989
14
1647.599976
37.166668
1249.609985
9.033334
0.208149812
15
1046.560059
23.299999
1071.859985
0
-0.036872194
16
1076.910034
0
943.26001
0
0.243297541
17
1512.209961
1.433333
1098.310059
5.733333
0.329645213
18
1545.550049
8.633333
1337.589966
0.633333
0.034863083
19
620.429993
13.833333
577.429993
0.966667
0.482295954
20
798.179993
0
777.559998
16.633333
0.35043662
17328 852 Kbytes .Supplementary data files not provided
