|
| Status |
Public on Feb 29, 2016 |
| Title |
Xenopus laevis expression data from larval inner ear tissue s56_1 |
| Sample type |
RNA |
| |
|
| Source name |
Xenopus laevis larvae
|
| Organism |
Xenopus laevis |
| Characteristics |
tissue: inner ear
developmental stage: larval stages 56-58
age: larval animals 38-44 days post-fertilization
|
| Growth protocol |
Larval Xenopus laevis (n=10) were aged 38-44 days post-fertilization with an average weight of 0.55 ± 0.11 g and an average length of 5.03 ± 0.30 cm and obtained from Nasco (Fort Atkinson, WI). Animal procedures and protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of New Mexico State University.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Valencia, CA) as per Trujillo-Provencio C, Powers T, Sultemeier D, Serrano E: RNA isolation from Xenopus inner ear sensory endorgans for transcriptional profiling and molecular cloning. Methods Mol Biol 2009, 493:3-20.
|
| Label |
biotin
|
| Label protocol |
Labeled antisense single-stranded cDNA was prepared from each RNA replicate (1 ug total RNA) using the Ovation RNA Amplification System V2 in conjunction with the FL-Ovation cDNA Biotin Module V2 (both from NuGEN)
|
| |
|
| Hybridization protocol |
Fragmented cDNA was hybridized according to manufacture's protocol (Affymetrix)
|
| Scan protocol |
GeneChip Xenopus laevis 2.0 were scanned using the GeneChip Scanner 3000 7G (Affymetrix)
|
| Description |
Gene expression data from larval animals ranging from stage 56 to stage 58 based on Nieuwkoop and Faber (1994) Normal Table of Xenopus laevis (Daudin). Garland Publishing Inc, New York ISBN 0-8153-1896-0.
|
| Data processing |
Data processed using JMP Genomics software
|
| |
|
| Submission date |
Oct 07, 2015 |
| Last update date |
Feb 29, 2016 |
| Contact name |
Selene Virk |
| Organization name |
Case Western Reserve University
|
| Street address |
2103 Cornell Road
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106-7281 |
| Country |
USA |
| |
|
| Platform ID |
GPL10756 |
| Series (1) |
| GSE73829 |
Inner ear expression data from pooled inner ear tissue from stages 56,57,58 Xenopus laevis larval inner ears |
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