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Status
Public on Mar 23, 2004
Title
267B1 vs. PC3M. Array 3.
Sample type
genomic
Channel 1 Source name
prostate cell line 267B1 DNA, Cy3
Organism
Homo sapiens Extracted molecule
genomic DNA
Channel 2 Source name
prostate cell line PC3M DNA, Cy5
Organism
Homo sapiens Extracted molecule
genomic DNA
Description
The methylation status of 267B1 and PC3M are compared. DNA samples were digested with methylation sensitive enzyme HpaII, ligated with adaptor, PCR amplified and labeled with Cye Dye. The labeled target are hybridized with human promoter array. In general, methylated sequences will not be cut and will not be amplified. Only unmethylated sequences will be amplified and hybridized to arrays.
Submission date
Mar 20, 2004
Last update date
May 27, 2005
Contact name
Yipeng Wang
E-mail(s)
ywang@sdibr.org Phone
8585813960
Organization name
Vaccine Research Institute of San Diego
Street address
City
San Diego
State/province
CA
ZIP/Postal code
92121
Country
USA
Platform ID
GPL864 Series (1)
GSE951 267B1, PC3M, LNCaP Methylation Profile vs. mRNA Expression Level
Data table header descriptions ID_REF
CH1_RAW Intensity of spots. 267B1, Cy3.
CH1_BKD_RAW Intensity of spots background.
CH2_RAW Intensity of spots. PC3M, Cy5.
CH2_BKD_RAW Intensity of spots background.
VALUE Lowess normalized log base 2 ratio (Ch2/Ch1).
Data table ID_REF CH1_RAW CH1_BKD_RAW CH2_RAW CH2_BKD_RAW VALUE 1
338.019989
0
486.880005
0.7
0.055327738
2
323.369995
0
621.900024
0
-0.333440641
3
368.140015
0
643.52002
0
-0.218244274
4
11894.7002
0
9018
0
-0.080487649
5
836.059998
0
1043.51001
0
-0.002959032
6
268.829987
0
539.51001
0
-0.404942406
7
1591.51001
0
1815.569946
70.566666
-0.090477227
8
367.410004
0
952.559998
49.833332
-0.735010994
9
548.049988
0
696.909973
3.1
0.160825236
10
596.659973
0
929.900024
0
-0.181341384
11
269.899994
8.333333
829.849976
16.200001
-0.993273418
12
1139.150024
0
1214.060059
0
0.079085041
13
1367.98999
0
1921.680054
15.766666
-0.40928265
14
1793.170044
0
1754.22998
0
0.048142153
15
1253.48999
0
1269.810059
0
0.114478376
16
837.049988
0
958.179993
13.4
0.164248486
17
1161.420044
0
1310.73999
0
-0.021243744
18
1508.23999
0
1382.650024
0
0.195025093
19
465.279999
0
516.619995
60.200001
0.582893414
20
756.690002
0
674.630005
5.9
0.611196057
17328 797 Kbytes .Supplementary data files not provided
