|
Status |
Public on Jun 02, 2017 |
Title |
Sorted Gr-1intermediate/Mac-1+ CEBPe-KO replicate 2 |
Sample type |
RNA |
|
|
Source name |
Sorted Gr-1intermediate/Mac-1+ cells
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 genotype: CEBPe KO cell type: Sorted primary Gr-1 intermediate/Mac-1+ bone marrow cells
|
Growth protocol |
sorted primary C57BL/6 (WT and CEBPe KO) bone marrow cells without culture
|
Extracted molecule |
total RNA |
Extraction protocol |
Rneasy Kit (Qiagen)
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 2
|
Data processing |
Data were normalized by applying control background correction, log transformation and inter-quantile normalization using the neqc function from the limma bioconductor package.
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|
|
Submission date |
Oct 08, 2015 |
Last update date |
Jun 02, 2017 |
Contact name |
Touati Benoukraf |
E-mail(s) |
tbenoukraf@mun.ca
|
Phone |
+1 (709) 864-6671
|
Organization name |
Memorial University of Newfoundland
|
Department |
Faculty of Medicine, Division of BioMedical Sciences
|
Street address |
Craig L. Dobbin Genetics Research Centre,
|
City |
St. John's |
State/province |
NL |
ZIP/Postal code |
A1B 3V6 |
Country |
Canada |
|
|
Platform ID |
GPL6887 |
Series (2) |
GSE73835 |
The effect of C/EBPepsilon in differentiated granulocytes: cDNA microarray of C/EBPepsilon knock out mice vs C57BL/6 mice bone marrow cells |
GSE73860 |
Landscape of CEBPe target genes in mouse bone marrow |
|