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Status
Public on Mar 23, 2004
Title
267B1 vs. PC3M. Array 4
Sample type
genomic
Channel 1 Source name
prostate cell line 267B1 DNA, Cy5
Organism
Homo sapiens Extracted molecule
genomic DNA
Channel 2 Source name
prostate cell line PC3M DNA, Cy3
Organism
Homo sapiens Extracted molecule
genomic DNA
Description
The methylation status of 267B1 and PC3M are compared. DNA samples were digested with methylation sensitive enzyme HpaII, ligated with adaptor, PCR amplified and labeled with Cye Dye. The labeled target are hybridized with human promoter array. In general, methylated sequences will not be cut and will not be amplified. Only unmethylated sequences will be amplified and hybridized to arrays.
Submission date
Mar 20, 2004
Last update date
May 27, 2005
Contact name
Yipeng Wang
E-mail(s)
ywang@sdibr.org Phone
8585813960
Organization name
Vaccine Research Institute of San Diego
Street address
City
San Diego
State/province
CA
ZIP/Postal code
92121
Country
USA
Platform ID
GPL864 Series (1)
GSE951 267B1, PC3M, LNCaP Methylation Profile vs. mRNA Expression Level
Data table header descriptions ID_REF
CH1_RAW Intensity of spots. 267B1, Cy5
CH1_BKD_RAW Intensity of spots background.
CH2_RAW Intensity of spots. PC3M, Cy3
CH2_BKD_RAW Intensity of spots background.
VALUE Lowess normalized log base 2 ratio (Ch2/Ch1).
Data table ID_REF CH1_RAW CH1_BKD_RAW CH2_RAW CH2_BKD_RAW VALUE 1
626.580017
9.6
416.369995
0
-0.284273561
2
551.5
0
351.059998
0
-0.260444865
3
722.51001
42.266666
371.390015
0
0.0116894
4
5881.660156
42.233334
6601.359863
0
-0.225661386
5
921.080017
4.133333
792.219971
0
-0.377001536
6
718.820007
49.866665
378.290009
0
-0.03771553
7
1228.839966
7.666667
922.72998
0
-0.119129837
8
708.210022
0
323.700012
7.2
0.26548026
9
800.690002
0
530.710022
0
-0.050887132
10
926.609985
1.4
374.799988
0
0.558487715
11
510.200012
47.433334
369.950012
0
-0.605607021
12
1017.890015
10
582.01001
0
0.166341892
13
1182.73999
6.933333
902.309998
0
-0.155482555
14
1526.790039
2.6
1165.22998
0
-0.018728615
15
1465.959961
35.400002
885.130005
0
0.1953183
16
870.679993
6.3
543.690002
0
0.039636798
17
1119.390015
24.5
648.460022
0
0.154896829
18
1156.589966
11.9
580.549988
0
0.361614078
19
667.309998
2.8
571.809998
0
-0.471314218
20
1046.869995
11.566667
760.340027
0
-0.136100899
17328 801 Kbytes .Supplementary data files not provided
