|
Status |
Public on Oct 01, 2016 |
Title |
Liver_GMO+R rep6 |
Sample type |
RNA |
|
|
Source name |
Liver_GMO+R
|
Organism |
Rattus norvegicus |
Characteristics |
gender: female strain: Sprague-Dawley tissue: Liver experimental group: GMO+R age at death (days): 722
|
Treatment protocol |
Livers were extracted at autopsy and half of the organ was snap frozen for RNA subsequent RNA isolation. Samples were stored at -80 until extraction.
|
Extracted molecule |
total RNA |
Extraction protocol |
Frozen liver samples were homogenised and RNA extracted using MagMAX-96 for Micorarrays Kit (AM1839). The spin procedure was followed. RNA was eluted in 50ul of elution buffer.
|
Label |
Biotin
|
Label protocol |
RNA was processed using the Ambion WT Expression kit and Affymetrix WT Terminal Labelling kit.
|
|
|
Hybridization protocol |
Hybridisation cocktails were prepared using 5.5ug cDNA as described in the Eukaryotic Array manual from Affymetrix, using the Hybridisation, Wash and Stain (HWS) kit (Affymetrix)
|
Scan protocol |
Arrays were scanned using the GCS3000 7G scanner (Affymetrix) following manufacturer's protocols.
|
Data processing |
CEL files were imported into Expression Console software (Affymetrix) and normalised using RMA-sketch (gene-level).
|
|
|
Submission date |
Oct 09, 2015 |
Last update date |
Oct 01, 2016 |
Contact name |
Matthew Arno |
E-mail(s) |
matthew.arno@kcl.ac.uk
|
Phone |
442078484286
|
Organization name |
King's College London
|
Department |
Genomics Centre
|
Lab |
Arno
|
Street address |
150 Stamford Street
|
City |
London |
State/province |
LONDON |
ZIP/Postal code |
SE1 9NH |
Country |
United Kingdom |
|
|
Platform ID |
GPL17117 |
Series (2) |
GSE73886 |
Transcriptome analysis of liver and kidneys of rats chronically fed a NK603 Roundup-tolerant genetically modified maize [liver] |
GSE73888 |
Transcriptome analysis of liver and kidneys of rats chronically fed a NK603 Roundup-tolerant genetically modified maize |
|