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Sample GSM1908970 Query DataSets for GSM1908970
Status Public on Jun 12, 2017
Title -10°C eWAT,biological rep3
Sample type RNA
 
Source name epididymal adipose tissue from mice exposed to -10°C
Organism Mus musculus
Characteristics tissue: epididymal adipose tissue
Treatment protocol Epididymal adipose tissue was washed by cold RNase-free normal saline, quickly dissected and put in liquid nitrogen when sacrificing the mice. Tissues were stored in -80°C until RNA extraction.
Growth protocol Epididymal adipose tissue from mice exposed to 30°C, 4°C, -10°C and -20°C.
Extracted molecule total RNA
Extraction protocol All samples were prepared using Trizol. Total RNA was isolated using the RNeasy Mini Kit (QIAGEN), cDNA synthesis and amplification was performed using the WT cDNA synthesis and amplification kit (Affymetrix).
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA by using Ambion® WT Expression Kit.
 
Hybridization protocol Following labeling, 5.5 ug of cDNA were hybridized for 16 hr at 45°C on GeneChip Mouse Exon 1.0 in Hybridization Oven 645. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol Affymetrix Gene ChIP Scanner 3000 7G
Description Gene expression data from mice epididymal adipose tissue exposed to -10°C
Data processing The data were analyzed with Robust Multichip Average (RMA) using Affymetrix Expression Console default analysis settings and global scaling as normalization method. Normalized signal intensities were transformed to the log base 2 scale.
 
Submission date Oct 15, 2015
Last update date Jun 12, 2017
Contact name Xiaoyan Yang
Organization name Shandong University Qilu Hospital
Street address 107 West Wenhua Road
City Jinan
ZIP/Postal code 250012
Country China
 
Platform ID GPL6096
Series (1)
GSE74062 Expression data from mice epididymal adipose tissue

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
6747308 8.616556
6747309 7.596976
6747314 7.850509
6747326 2.303027
6747343 7.655443
6747354 2.467376
6747364 9.384077
6747471 7.06285
6747472 10.31671
6747478 2.867655
6747497 7.328987
6747504 2.422402
6747515 6.759686
6747577 5.129993
6747641 8.472034
6747696 2.1003
6747786 6.029056
6747805 8.284306
6747837 8.752399
6747839 8.849526

Total number of rows: 23332

Table truncated, full table size 384 Kbytes.




Supplementary file Size Download File type/resource
GSM1908970_minus10C_eWAT-2.CEL.gz 21.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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