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Sample GSM19282 Query DataSets for GSM19282
Status Public on Jul 01, 2004
Title CEA_SGF:AGQ031
Sample type RNA
 
Channel 1
Source name Tet OFF 16h (Cy5)
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Reference 0h (Cy3)
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Tet OFF16h ARN test 4x105 cells were plated in a 10 cm cell culture plate and cultured in the presence of LIF 1000U/ml but without tetracycline for 24h to allow expression of STAT3F (Tet OFF 16h). Cells were then trypsinized and total RNA was extracted.
ARN ref 4x105 cells were plated in a 10 cm cell culture plate and cultured under standard condition (+LIF 1000 U/ml, tetracycline 1µg/ml) for 48h.
Lot batch = CEA_SGF:AGQ
 
Submission date Mar 26, 2004
Last update date Oct 28, 2005
Contact name Dalila Sekkai
E-mail(s) dsekkai@igr.fr
Phone +33 (0)1 42 11 49 19
Organization name IGR
Department hematopoiesis and stem cell
Lab INSERM U362
Street address
City Villejuif
ZIP/Postal code 94805
Country France
 
Platform ID GPL1063
Series (1)
GSE1151 CEA_SGF:E00004#TetOFF_Stat3F

Data table header descriptions
ID_REF
VALUE log2 ratio of the median intensities of each feature for each wavelength, with the median background not subtracted.
DIA the diameter in µm of the feature-indicator.
F635_Median median feature pixel intensity at wavelength #1 (635 nm).
F635_Mean mean feature pixel intensity at wavelength #1 (635 nm).
F635_SD the standard deviation of the feature pixel intensity at wavelength #1 (635 nm).
B635_Median the median feature background intensity at wavelength #1 (635 nm).
B635_Mean the mean feature background intensity at wavelength #1 (635 nm).
B635_SD the standard deviation of the feature background intensity at wavelength #1 (635 nm).
%_>_B635+1SD the percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength #1 (635 nm).
%_>_B635+2SD the percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength #1 (635 nm).
F635_%_Sat the percentage of feature pixels at wavelength #1 that are saturated.
F532_Median median feature pixel intensity at wavelength #2 (532 nm).
F532_Mean mean feature pixel intensity at wavelength #2 (532 nm).
F532_SD the standard deviation of the feature intensity at wavelength #2 (532 nm).
B532_Median the median feature background intensity at wavelength #2 (532 nm).
B532_Mean the mean feature background intensity at wavelength #2 (532 nm).
B532_SD the standard deviation of the feature background intensity at wavelength #2 (532 nm).
%_>_B532+1SD the percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength #2 (532 nm).
%_>_B532+2SD the percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength #2 (532 nm).
F532_%_Sat the percentage of feature pixels at wavelength #2 that are saturated.
Ratio_of_Medians_(635/532) the ratio of the median intensities of each feature for each wavelength, with the median background subtracted.
Ratio_of_Means_(635/532) the ratio of the arithmetic mean intensities of each feature for each wavelength, with the median background subtracted.
Median_of_Ratios_(635/532) the median of pixel-by-pixel ratios of pixel intensities, with the median background subtracted.
Mean_of_Ratios_(635/532) the geometric mean of the pixel-by-pixel ratios of pixel intensities, with the median background subtracted.
Ratios_SD_(635/532) the geometric standard deviation of the pixel intensity ratios.
Rgn_Ratio_(635/532) the regression ratio of every pixel in a 2-feature-diameter circle around the center of the feature.
Rgn_R²_(635/532) the coefficient of determination for the current regression value.
Flags the type of flag associated with a feature.

Data table
ID_REF VALUE DIA F635_Median F635_Mean F635_SD B635_Median B635_Mean B635_SD %_>_B635+1SD %_>_B635+2SD F635_%_Sat F532_Median F532_Mean F532_SD B532_Median B532_Mean B532_SD %_>_B532+1SD %_>_B532+2SD F532_%_Sat Ratio_of_Medians_(635/532) Ratio_of_Means_(635/532) Median_of_Ratios_(635/532) Mean_of_Ratios_(635/532) Ratios_SD_(635/532) Rgn_Ratio_(635/532) Rgn_R²_(635/532) Flags
1.1.1 -0.76 80 201 202 68 84 90 33 92 71 0 341 334 67 289 293 48 51 21 0 2.268 2.599 1.557 1.786 2.486 2.036 0.018 0
1.2.1 -0.86 120 189 200 73 83 90 32 89 69 0 342 354 75 288 291 40 60 39 0 1.979 1.772 1.211 1.425 2.789 1.687 0.073 0
1.3.1 -1.04 130 375 384 138 85 93 45 96 96 0 769 754 212 292 298 49 99 97 0 0.609 0.647 0.638 0.637 1.854 0.658 0.57 0
1.4.1 -1.52 160 93 104 43 84 160 1014 0 0 0 267 274 43 306 391 1201 0 0 0 -0.226 -0.615 0.421 0.489 4.94 0.664 0.662 -50
1.5.1 -2.57 180 123 139 76 85 127 672 0 0 0 730 784 344 381 470 577 26 2 0 0.109 0.133 0.148 0.146 2.714 0.033 0.001 0
1.6.1 -2.36 180 285 354 251 87 117 507 21 0 0 1467 1933 1583 494 723 733 61 36 0 0.203 0.185 0.209 0.193 2.593 0.085 0.015 0
1.7.1 -2.52 160 108 127 63 85 93 42 37 21 0 620 658 176 479 545 361 17 1 0 0.159 0.234 0.208 0.198 3.138 0.092 0.1 -50
1.8.1 -1.61 100 608 640 249 88 101 58 100 97 0 1860 1896 556 466 478 173 100 98 0 0.373 0.386 0.379 0.375 1.498 0.358 0.762 0
1.9.1 -2.47 160 92 97 51 86 92 33 15 3 0 511 519 96 469 459 152 12 1 0 0.139 0.214 0.3 0.284 4.24 0.041 0.013 -50
1.10.1 -2.51 160 89 92 31 87 91 30 14 3 0 507 507 78 449 442 131 12 1 0 0.034 0.085 0.272 0.28 4.011 0.012 0.002 -50
1.11.1 -2.12 160 91 100 45 86 91 30 20 7 0 396 415 107 438 433 133 8 1 0 -0.116 -0.606 0.199 0.233 3.903 0.008 0.001 -50
1.12.1 -2.07 160 89 98 45 86 92 30 20 6 0 374 395 128 415 430 136 3 1 0 -0.071 -0.572 0.299 0.357 4.439 0.038 0.012 -50
1.13.1 -2.53 160 86 93 34 87 92 33 14 5 0 496 490 85 478 474 159 3 0 0 -0.053 0.476 0.323 0.313 3.67 0.02 0.005 -50
1.14.1 -1.61 140 329 335 92 88 94 34 100 100 0 1004 988 170 517 503 139 96 87 0 0.495 0.524 0.49 0.529 1.683 0.372 0.511 0
1.15.1 -2.19 150 332 338 98 88 94 35 99 97 0 1514 1502 208 541 545 102 100 100 0 0.251 0.26 0.259 0.243 1.56 0.247 0.687 0
1.16.1 -2.52 160 92 97 31 88 94 36 14 5 0 526 541 106 497 509 83 25 8 0 0.136 0.199 0.373 0.377 3.712 0.006 0 -50
1.1.2 -0.50 160 861 832 326 84 89 32 99 99 0 1216 1190 413 301 306 60 99 98 0 0.847 0.841 0.859 0.864 1.611 0.816 0.819 0
1.2.2 -0.40 160 745 715 272 85 92 34 99 99 0 982 961 239 297 301 46 99 99 0 0.963 0.947 0.937 0.896 1.439 0.974 0.86 0
1.3.2 -0.09 160 2584 2294 950 85 93 39 100 99 0 2750 2484 893 300 306 51 100 100 0 1.02 1.011 1.008 0.991 1.456 1.024 0.921 0
1.4.2 -0.54 160 1857 1755 673 88 103 64 99 98 0 2705 2498 814 315 332 90 100 99 0 0.74 0.763 0.752 0.732 1.582 0.764 0.898 0

Total number of rows: 9216

Table truncated, full table size 1115 Kbytes.




Supplementary data files not provided
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