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Sample GSM19290 Query DataSets for GSM19290
Status Public on Jul 01, 2004
Title CEA_SGF:AGQ030
Sample type RNA
 
Channel 1
Source name Reference 0h (Cy5)
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Tet OFF 48h (Cy3)
Organism Mus musculus
Extracted molecule total RNA
 
 
Description ARN ref 4x105 cells were plated in a 10 cm cell culture plate and cultured under standard condition (+LIF 1000 U/ml, tetracycline 1µg/ml).
Tet OFF48h ARN test 4x105 cells were plated in a 10 cm cell culture plate and cultured in the presence of LIF 1000U/ml but without tetracycline for 48h to allow expression of STAT3F (Tet OFF 48h). Cells were then trypsinized and total RNA was extracted.
Lot batch = CEA_SGF:AGQ
 
Submission date Mar 26, 2004
Last update date Oct 28, 2005
Contact name Dalila Sekkai
E-mail(s) dsekkai@igr.fr
Phone +33 (0)1 42 11 49 19
Organization name IGR
Department hematopoiesis and stem cell
Lab INSERM U362
Street address
City Villejuif
ZIP/Postal code 94805
Country France
 
Platform ID GPL1063
Series (1)
GSE1151 CEA_SGF:E00004#TetOFF_Stat3F

Data table header descriptions
ID_REF
VALUE log2 ratio of the median intensities of each feature for each wavelength, with the median background not subtracted.
DIA the diameter in µm of the feature-indicator.
F635_Median median feature pixel intensity at wavelength #1 (635 nm).
F635_Mean mean feature pixel intensity at wavelength #1 (635 nm).
F635_SD the standard deviation of the feature pixel intensity at wavelength #1 (635 nm).
B635_Median the median feature background intensity at wavelength #1 (635 nm).
B635_Mean the mean feature background intensity at wavelength #1 (635 nm).
B635_SD the standard deviation of the feature background intensity at wavelength #1 (635 nm).
%_>_B635+1SD the percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength #1 (635 nm).
%_>_B635+2SD the percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength #1 (635 nm).
F635_%_Sat the percentage of feature pixels at wavelength #1 that are saturated.
F532_Median median feature pixel intensity at wavelength #2 (532 nm).
F532_Mean mean feature pixel intensity at wavelength #2 (532 nm).
F532_SD the standard deviation of the feature intensity at wavelength #2 (532 nm).
B532_Median the median feature background intensity at wavelength #2 (532 nm).
B532_Mean the mean feature background intensity at wavelength #2 (532 nm).
B532_SD the standard deviation of the feature background intensity at wavelength #2 (532 nm).
%_>_B532+1SD the percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength #2 (532 nm).
%_>_B532+2SD the percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength #2 (532 nm).
F532_%_Sat the percentage of feature pixels at wavelength #2 that are saturated.
Ratio_of_Medians_(635/532) the ratio of the median intensities of each feature for each wavelength, with the median background subtracted.
Ratio_of_Means_(635/532) the ratio of the arithmetic mean intensities of each feature for each wavelength, with the median background subtracted.
Median_of_Ratios_(635/532) the median of pixel-by-pixel ratios of pixel intensities, with the median background subtracted.
Mean_of_Ratios_(635/532) the geometric mean of the pixel-by-pixel ratios of pixel intensities, with the median background subtracted.
Ratios_SD_(635/532) the geometric standard deviation of the pixel intensity ratios.
Rgn_Ratio_(635/532) the regression ratio of every pixel in a 2-feature-diameter circle around the center of the feature.
Rgn_R²_(635/532) the coefficient of determination for the current regression value.
Flags the type of flag associated with a feature.

Data table
ID_REF VALUE DIA F635_Median F635_Mean F635_SD B635_Median B635_Mean B635_SD %_>_B635+1SD %_>_B635+2SD F635_%_Sat F532_Median F532_Mean F532_SD B532_Median B532_Mean B532_SD %_>_B532+1SD %_>_B532+2SD F532_%_Sat Ratio_of_Medians_(635/532) Ratio_of_Means_(635/532) Median_of_Ratios_(635/532) Mean_of_Ratios_(635/532) Ratios_SD_(635/532) Rgn_Ratio_(635/532) Rgn_R²_(635/532) Flags
1.1.1 -0.51 130 479 490 158 95 109 69 100 97 0 684 709 219 258 267 58 100 99 0 0.903 0.876 0.883 0.913 1.348 0.854 0.676 0
1.2.1 -0.46 130 1026 1180 543 96 111 61 100 100 0 1407 1586 646 262 269 56 100 100 0 0.812 0.818 0.831 0.818 1.143 0.828 0.9 0
1.3.1 -0.63 140 861 809 329 94 107 51 98 96 0 1332 1264 408 259 265 52 100 100 0 0.715 0.712 0.69 0.658 1.449 0.731 0.828 0
1.4.1 -0.96 160 144 151 64 99 112 53 44 20 0 281 287 58 258 265 54 27 9 0 2.025 1.776 1.21 1.321 2.62 2.824 0.024 -50
1.5.1 -0.75 150 568 599 192 106 120 58 100 100 0 955 982 268 269 281 63 99 98 0 0.675 0.693 0.689 0.697 1.303 0.692 0.759 0
1.6.1 -0.09 150 2801 2985 1602 109 124 62 100 100 0 2980 3305 1689 272 285 67 100 100 0 0.994 0.948 0.963 0.943 1.054 0.957 0.948 0
1.7.1 -0.53 130 620 617 186 108 123 59 100 100 0 896 888 204 269 281 62 100 99 0 0.816 0.822 0.805 0.817 1.243 0.846 0.726 0
1.8.1 -0.48 130 1265 1311 541 113 127 63 100 100 0 1759 1766 603 264 273 63 100 100 0 0.77 0.798 0.768 0.774 1.099 0.817 0.898 0
1.9.1 -1.07 160 119 133 63 110 127 67 17 7 0 249 255 58 257 271 66 10 2 0 -1.157 -10.127 0.945 0.782 3.535 10.362 0.002 -50
1.10.1 -1.29 160 102 119 54 106 123 60 16 5 0 249 265 88 258 272 68 12 6 0 0.405 2.025 0.746 0.812 3.466 0.292 0.018 -50
1.11.1 -1.25 160 108 133 72 111 132 94 15 3 0 256 266 65 266 282 106 6 0 0 0.36 81015.9 0.768 0.856 3.491 4.338 0.01 -50
1.12.1 -1.57 80 102 126 72 110 132 72 17 7 0 303 739 1184 268 295 103 38 26 0 -0.235 0.032 0.573 0.506 4.952 0.008 0.004 0
1.13.1 -1.16 160 112 131 81 115 130 63 14 6 0 251 269 78 261 276 75 15 7 0 0.27 1.823 0.81 0.767 3.204 2.149 0.02 -50
1.14.1 -0.76 150 1561 1554 316 121 138 77 100 100 0 2646 2625 414 266 281 76 100 100 0 0.605 0.608 0.611 0.6 1.009 0.612 0.901 0
1.15.1 -2.95 160 493 492 157 121 140 81 94 89 0 3814 3620 981 272 284 69 100 99 0 0.105 0.111 0.107 0.104 1.317 0.103 0.751 0
1.16.1 -1.23 160 117 128 54 111 127 63 20 4 0 275 287 82 266 279 68 16 5 0 0.709 0.81 0.972 0.931 3.242 0.865 0.018 -50
1.1.2 -0.35 140 2493 2361 702 92 106 64 100 100 0 3177 3028 722 257 265 55 100 100 0 0.822 0.819 0.798 0.797 1.091 0.837 0.913 0
1.2.2 -0.21 150 1396 1370 292 96 110 65 100 100 0 1616 1565 321 262 269 56 100 99 0 0.96 0.978 0.992 0.996 1.058 0.969 0.916 0
1.3.2 -0.41 150 5295 5257 796 94 108 54 100 100 0 7045 6974 822 258 266 53 100 100 0 0.766 0.769 0.778 0.763 0.97 0.776 0.94 0
1.4.2 -0.20 150 5176 5024 949 97 109 53 100 100 0 5961 5807 887 257 266 55 100 100 0 0.89 0.888 0.896 0.876 1.048 0.902 0.929 0

Total number of rows: 9216

Table truncated, full table size 1120 Kbytes.




Supplementary data files not provided
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