|
| Status |
Public on Oct 23, 2018 |
| Title |
Brain_8w_BS |
| Sample type |
SRA |
| |
|
| Source name |
8-week-old Mouse brain
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BJ/6
gender: male
transfection: none
age: 8 weeks
tissue: brain
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
mRNAs of 8-week-old mouse brain were purified using oligo (dT)-conjugated magnetic beads followed by RiboMinus treatement.
Bisulfite treated mRNAs were reverse transcribed to cDNA using ACT random hexamer primers. The cDNAs were subjected to libraries construction using KAPA Stranded mRNA-Seq Kit (KAPA) and performed sequencing on HiSeq2000 (Illumina) in pair-end mode, creating reads with a length of 101 bp.
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
mRNA m5C profiles in 8-week-old mouse brain
|
| Data processing |
Raw Reads were stripped of adaptor sequences and low quality bases were removed using Trimmomatic. Filtered paired-end RNA-BisSeq reads were mapped against mouse (mm10) reference genome by Bismark (version 0.13.0). The m5C sites calling was performed using Bismark (version 0.13.0).
Genome_build: mm10
Supplementary_files_format_and_content: txt format. Information of m5C positions and modified gene types (mm10).
|
| |
|
| Submission date |
Nov 06, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Bao-Fa Sun |
| E-mail(s) |
sunbf@big.ac.cn
|
| Organization name |
Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
|
| Street address |
Da-Tun Road
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE74771 |
Single-base resolution methylome of 5-methylcytosine in mammalian transcriptome |
|
| Relations |
| BioSample |
SAMN04247343 |
| SRA |
SRX1422965 |
