|
Status |
Public on Nov 10, 2015 |
Title |
A00026868 |
Sample type |
RNA |
|
|
Source name |
vastus lateralis
|
Organism |
Homo sapiens |
Characteristics |
response to exercise: responder phenotype: obese disease state: Type 2 diabetes
|
Treatment protocol |
resting skeletal muscle
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from ~20mg of skeletal muscle tissue using the RNeasy Fibrous Tissue kit (Qiagen)
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Illumina Total Prep RNA Amplification Kit
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
obese, T2D
|
Data processing |
Data were normalized by quantile normalization, multiple imputation and log2-transformation, using SAS 9.2. Genes with high background intensity across all samples were filtered.
|
|
|
Submission date |
Nov 06, 2015 |
Last update date |
Nov 10, 2015 |
Contact name |
Lauren M. Sparks |
E-mail(s) |
lauren.sparks@flhosp.org
|
Organization name |
FLORIDA HOSPITAL
|
Department |
Translational Research Institute for Metabolism and Diabetes
|
Street address |
301 E. Princeton St
|
City |
Orlando |
State/province |
FL |
ZIP/Postal code |
32804 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE74782 |
Near-whole-genome transcriptome analysis of gene expression in human skeletal muscle tissue at baseline in obese individuals with Type 2 Diabetes |
|