|
| Status |
Public on Nov 10, 2015 |
| Title |
A00026868 |
| Sample type |
RNA |
| |
|
| Source name |
vastus lateralis
|
| Organism |
Homo sapiens |
| Characteristics |
response to exercise: responder
phenotype: obese
disease state: Type 2 diabetes
|
| Treatment protocol |
resting skeletal muscle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from ~20mg of skeletal muscle tissue using the RNeasy Fibrous Tissue kit (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina Total Prep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
obese, T2D
|
| Data processing |
Data were normalized by quantile normalization, multiple imputation and log2-transformation, using SAS 9.2. Genes with high background intensity across all samples were filtered.
|
| |
|
| Submission date |
Nov 06, 2015 |
| Last update date |
Nov 10, 2015 |
| Contact name |
Lauren M. Sparks |
| E-mail(s) |
lauren.sparks@flhosp.org
|
| Organization name |
FLORIDA HOSPITAL
|
| Department |
Translational Research Institute for Metabolism and Diabetes
|
| Street address |
301 E. Princeton St
|
| City |
Orlando |
| State/province |
FL |
| ZIP/Postal code |
32804 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE74782 |
Near-whole-genome transcriptome analysis of gene expression in human skeletal muscle tissue at baseline in obese individuals with Type 2 Diabetes |
|
