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Sample GSM19359 Query DataSets for GSM19359
Status Public on Jul 01, 2004
Title CEA_SGF:AHU012
Sample type RNA
 
Channel 1
Source name Tet OFF 24h (Cy5)
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Reference 0h (Cy3)
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Tet OFF24h ARN test 4x105 cells were plated in a 10 cm cell culture plate and cultured in the presence of LIF 1000U/ml but without tetracycline for 24h to allow expression of STAT3F (Tet OFF 24h). Cells were then trypsinized and total RNA was extracted.
ARN ref 4x105 cells were plated in a 10 cm cell culture plate and cultured under standard condition (+LIF 1000 U/ml, tetracycline 1µg/ml) for 48h.
Lot batch = CEA_SGF:AHU
 
Submission date Mar 29, 2004
Last update date Oct 28, 2005
Contact name Dalila Sekkai
E-mail(s) dsekkai@igr.fr
Phone +33 (0)1 42 11 49 19
Organization name IGR
Department hematopoiesis and stem cell
Lab INSERM U362
Street address
City Villejuif
ZIP/Postal code 94805
Country France
 
Platform ID GPL1091
Series (1)
GSE1151 CEA_SGF:E00004#TetOFF_Stat3F

Data table header descriptions
ID_REF
VALUE log2 ratio of the median intensities of each feature for each wavelength, with the median background not subtracted.
DIA the diameter in µm of the feature-indicator.
F635_Median median feature pixel intensity at wavelength #1 (635 nm).
F635_Mean mean feature pixel intensity at wavelength #1 (635 nm).
F635_SD the standard deviation of the feature pixel intensity at wavelength #1 (635 nm).
B635_Median the median feature background intensity at wavelength #1 (635 nm).
B635_Mean the mean feature background intensity at wavelength #1 (635 nm).
B635_SD the standard deviation of the feature background intensity at wavelength #1 (635 nm).
%_>_B635+1SD the percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength #1 (635 nm).
%_>_B635+2SD the percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength #1 (635 nm).
F635_%_Sat the percentage of feature pixels at wavelength #1 that are saturated.
F532_Median median feature pixel intensity at wavelength #2 (532 nm).
F532_Mean mean feature pixel intensity at wavelength #2 (532 nm).
F532_SD the standard deviation of the feature intensity at wavelength #2 (532 nm).
B532_Median the median feature background intensity at wavelength #2 (532 nm).
B532_Mean the mean feature background intensity at wavelength #2 (532 nm).
B532_SD the standard deviation of the feature background intensity at wavelength #2 (532 nm).
%_>_B532+1SD the percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength #2 (532 nm).
%_>_B532+2SD the percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength #2 (532 nm).
F532_%_Sat the percentage of feature pixels at wavelength #2 that are saturated.
Ratio_of_Medians_(635/532) the ratio of the median intensities of each feature for each wavelength, with the median background subtracted.
Ratio_of_Means_(635/532) the ratio of the arithmetic mean intensities of each feature for each wavelength, with the median background subtracted.
Median_of_Ratios_(635/532) the median of pixel-by-pixel ratios of pixel intensities, with the median background subtracted.
Mean_of_Ratios_(635/532) the geometric mean of the pixel-by-pixel ratios of pixel intensities, with the median background subtracted.
Ratios_SD_(635/532) the geometric standard deviation of the pixel intensity ratios.
Rgn_Ratio_(635/532) the regression ratio of every pixel in a 2-feature-diameter circle around the center of the feature.
Rgn_R²_(635/532) the coefficient of determination for the current regression value.
Flags the type of flag associated with a feature.

Data table
ID_REF VALUE DIA F635_Median F635_Mean F635_SD B635_Median B635_Mean B635_SD %_>_B635+1SD %_>_B635+2SD F635_%_Sat F532_Median F532_Mean F532_SD B532_Median B532_Mean B532_SD %_>_B532+1SD %_>_B532+2SD F532_%_Sat Ratio_of_Medians_(635/532) Ratio_of_Means_(635/532) Median_of_Ratios_(635/532) Mean_of_Ratios_(635/532) Ratios_SD_(635/532) Rgn_Ratio_(635/532) Rgn_R²_(635/532) Flags
1.1.1 -0.79 90 985 1014 315 282 338 216 98 82 0 1706 1753 391 1622 1638 392 19 5 0 8.369 5.588 2 2.213 3.415 1.105 0.026 0
1.2.1 -0.07 120 2748 2679 783 258 279 135 100 100 0 2889 2867 646 895 1095 442 100 95 0 1.249 1.228 1.2 1.228 1.269 1.246 0.847 0
1.3.1 -0.28 130 1652 1677 477 274 293 120 100 100 0 2008 2080 429 749 785 241 100 98 0 1.095 1.054 1.031 1.052 1.416 1.046 0.775 0
1.4.1 -0.95 160 363 410 254 284 387 1634 0 0 0 701 783 535 694 815 1329 1 0 0 11.286 1.416 1.015 1.154 3.85 1.301 0.779 -50
1.5.1 -0.53 120 835 886 339 281 305 161 95 82 0 1209 1324 1033 695 744 277 80 42 0 1.078 0.962 1.169 1.149 2.384 0.125 0.105 0
1.6.1 -0.26 150 3334 3221 720 279 328 702 99 98 0 4006 3906 707 681 763 992 99 93 0 0.919 0.912 0.925 0.909 1.239 0.919 0.938 0
1.7.1 -0.91 110 631 656 181 271 301 166 87 55 0 1186 1216 247 673 701 162 98 82 0 0.702 0.709 0.781 0.689 2.154 0.834 0.364 0
1.8.1 -0.15 140 3333 3218 1400 243 262 113 100 100 0 3695 3829 1462 638 666 256 100 100 0 1.011 0.932 0.904 0.91 1.395 0.915 0.89 0
1.9.1 -1.37 160 235 248 98 249 279 221 3 0 0 607 617 121 641 679 407 1 0 0 0.412 0.042 0.944 0.921 4.32 0.349 0.24 -50
1.10.1 -1.43 160 239 250 96 254 402 1471 0 0 0 644 647 93 651 746 1248 0 0 0 2.143 1 0.832 0.952 3.841 1.711 0.594 -50
1.11.1 -1.30 160 235 246 120 251 382 1447 0 0 0 578 576 104 606 692 1238 0 0 0 0.571 0.167 0.98 0.91 4.018 1.451 0.455 -50
1.12.1 -1.21 160 249 264 103 240 260 128 17 2 0 575 576 84 569 577 99 13 1 0 1.5 3.429 1.219 1.181 3.966 1.633 0.086 -50
1.13.1 -1.18 160 239 258 100 236 252 109 15 3 0 542 557 195 559 564 85 13 2 0 -0.176 -11 0.894 0.846 4.382 0.152 0.005 -50
1.14.1 -0.47 150 716 805 384 238 259 239 81 49 0 989 1046 294 543 550 90 98 92 0 1.072 1.127 1.153 1.069 2.124 1.689 0.479 0
1.15.1 -0.11 130 722 750 245 243 262 107 95 90 0 778 806 172 528 537 79 90 75 0 1.916 1.824 1.875 1.955 2.429 1.907 0.501 0
1.16.1 -1.03 80 256 277 102 256 269 101 19 5 0 521 517 72 524 530 85 5 1 0 0 -3 0.881 1.023 4.176 2.669 0.019 0
1.1.2 -0.21 130 2128 2303 756 245 264 126 100 100 0 2462 2616 501 835 939 356 100 100 0 1.157 1.156 1.133 1.126 1.315 1.173 0.866 0
1.2.2 -0.64 140 1020 1088 366 259 275 115 100 98 0 1586 1657 339 874 1024 399 91 41 0 1.069 1.059 1.042 1.055 1.587 0.151 0.133 0
1.3.2 -0.36 150 2752 2808 739 275 335 861 99 86 0 3522 3754 818 829 983 1526 100 36 0 0.92 0.866 0.855 0.858 1.308 0.846 0.927 0
1.4.2 -0.25 150 2456 2730 1050 275 420 2008 60 10 0 2914 3038 854 741 878 1652 79 12 0 1.004 1.069 1.074 1.05 1.386 1.208 0.896 0

Total number of rows: 9216

Table truncated, full table size 1145 Kbytes.




Supplementary data files not provided
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