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Sample GSM19367 Query DataSets for GSM19367
Status Public on Jul 01, 2004
Title CEA_SGF:AHU025
Sample type RNA
 
Channel 1
Source name Reference 0h (Cy5)
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Tet OFF 24h (Cy3)
Organism Mus musculus
Extracted molecule total RNA
 
 
Description ARN ref 4x105 cells were plated in a 10 cm cell culture plate and cultured under standard condition (+LIF 1000 U/ml, tetracycline 1µg/ml) for 48h.
Tet OFF24h ARN test 4x105 cells were plated in a 10 cm cell culture plate and cultured in the presence of LIF 1000U/ml but without tetracycline for 24h to allow expression of STAT3F (Tet OFF 24h). Cells were then trypsinized and total RNA was extracted.
Lot batch = CEA_SGF:AHU
 
Submission date Mar 29, 2004
Last update date Oct 28, 2005
Contact name Dalila Sekkai
E-mail(s) dsekkai@igr.fr
Phone +33 (0)1 42 11 49 19
Organization name IGR
Department hematopoiesis and stem cell
Lab INSERM U362
Street address
City Villejuif
ZIP/Postal code 94805
Country France
 
Platform ID GPL1091
Series (1)
GSE1151 CEA_SGF:E00004#TetOFF_Stat3F

Data table header descriptions
ID_REF
VALUE log2 ratio of the median intensities of each feature for each wavelength, with the median background not subtracted.
DIA the diameter in µm of the feature-indicator.
F635_Median median feature pixel intensity at wavelength #1 (635 nm).
F635_Mean mean feature pixel intensity at wavelength #1 (635 nm).
F635_SD the standard deviation of the feature pixel intensity at wavelength #1 (635 nm).
B635_Median the median feature background intensity at wavelength #1 (635 nm).
B635_Mean the mean feature background intensity at wavelength #1 (635 nm).
B635_SD the standard deviation of the feature background intensity at wavelength #1 (635 nm).
%_>_B635+1SD the percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength #1 (635 nm).
%_>_B635+2SD the percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength #1 (635 nm).
F635_%_Sat the percentage of feature pixels at wavelength #1 that are saturated.
F532_Median median feature pixel intensity at wavelength #2 (532 nm).
F532_Mean mean feature pixel intensity at wavelength #2 (532 nm).
F532_SD the standard deviation of the feature intensity at wavelength #2 (532 nm).
B532_Median the median feature background intensity at wavelength #2 (532 nm).
B532_Mean the mean feature background intensity at wavelength #2 (532 nm).
B532_SD the standard deviation of the feature background intensity at wavelength #2 (532 nm).
%_>_B532+1SD the percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength #2 (532 nm).
%_>_B532+2SD the percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength #2 (532 nm).
F532_%_Sat the percentage of feature pixels at wavelength #2 that are saturated.
Ratio_of_Medians_(635/532) the ratio of the median intensities of each feature for each wavelength, with the median background subtracted.
Ratio_of_Means_(635/532) the ratio of the arithmetic mean intensities of each feature for each wavelength, with the median background subtracted.
Median_of_Ratios_(635/532) the median of pixel-by-pixel ratios of pixel intensities, with the median background subtracted.
Mean_of_Ratios_(635/532) the geometric mean of the pixel-by-pixel ratios of pixel intensities, with the median background subtracted.
Ratios_SD_(635/532) the geometric standard deviation of the pixel intensity ratios.
Rgn_Ratio_(635/532) the regression ratio of every pixel in a 2-feature-diameter circle around the center of the feature.
Rgn_R²_(635/532) the coefficient of determination for the current regression value.
Flags the type of flag associated with a feature.

Data table
ID_REF VALUE DIA F635_Median F635_Mean F635_SD B635_Median B635_Mean B635_SD %_>_B635+1SD %_>_B635+2SD F635_%_Sat F532_Median F532_Mean F532_SD B532_Median B532_Mean B532_SD %_>_B532+1SD %_>_B532+2SD F532_%_Sat Ratio_of_Medians_(635/532) Ratio_of_Means_(635/532) Median_of_Ratios_(635/532) Mean_of_Ratios_(635/532) Ratios_SD_(635/532) Rgn_Ratio_(635/532) Rgn_R²_(635/532) Flags
1.1.1 -1.05 120 614 693 309 187 243 670 27 2 0 1273 1322 294 989 1025 551 22 1 0 1.504 1.52 1.406 1.549 2.604 1.244 0.497 0
1.2.1 -0.34 120 1602 1614 840 187 246 674 74 52 0 2029 2074 594 989 1026 554 79 46 0 1.361 1.315 1.28 1.259 1.682 1.351 0.871 0
1.3.1 -0.51 130 1646 1664 501 189 204 89 100 100 0 2344 2317 531 983 989 132 99 98 0 1.071 1.106 1.146 1.15 1.651 1.07 0.765 0
1.4.1 -1.62 100 370 429 171 186 207 110 78 42 0 1137 1152 185 962 970 120 66 36 0 1.051 1.279 0.959 1.162 2.726 1.15 0.17 0
1.5.1 -0.79 140 999 1028 326 190 229 609 74 12 0 1728 1749 316 962 1001 541 82 15 0 1.056 1.065 1.135 1.094 1.772 1.026 0.651 0
1.6.1 0.20 130 5022 4964 825 189 236 657 100 100 0 4386 4422 618 963 1003 584 100 100 0 1.412 1.38 1.363 1.38 1.178 1.438 0.943 0
1.7.1 -1.11 110 641 652 214 192 256 799 7 0 0 1387 1412 229 966 973 165 90 72 0 1.067 1.031 1.002 1.045 2.129 0.965 0.374 0
1.8.1 -0.01 130 4219 4287 1047 194 216 103 100 100 0 4252 4204 956 955 963 122 100 100 0 1.221 1.26 1.257 1.273 1.309 1.244 0.905 0
1.9.1 -2.24 160 200 215 105 202 224 116 12 4 0 942 939 112 961 983 524 0 0 0 0.105 -0.591 0.617 0.647 4.235 0.059 0.13 -50
1.10.1 -2.21 160 206 222 109 205 224 105 15 3 0 956 957 128 956 981 545 0 0 0 100000 17 0.665 0.594 3.906 0.061 0.131 -50
1.11.1 -2.15 160 210 237 117 204 225 106 18 6 0 932 944 122 949 960 139 10 1 0 -0.353 -6.6 0.903 0.793 4.036 0.155 0.004 -50
1.12.1 -2.15 160 214 226 97 208 227 108 13 3 0 951 955 162 962 965 121 13 1 0 -0.545 -2.571 0.678 0.702 5.585 0.109 0.006 -50
1.13.1 -2.09 160 223 253 131 203 223 102 25 10 0 948 954 118 966 968 125 15 1 0 -1.111 -4.167 0.673 0.742 4.7 57.889 0 -50
1.14.1 -1.16 150 770 793 240 209 227 105 99 98 0 1721 1730 302 974 982 143 98 95 0 0.751 0.772 0.726 0.777 1.653 0.725 0.628 0
1.15.1 -1.75 140 480 486 166 212 243 291 41 5 0 1620 1627 281 962 980 174 96 88 0 0.407 0.412 0.395 0.375 2.202 0.368 0.389 0
1.16.1 -2.15 160 215 237 108 215 241 256 4 0 0 953 950 110 957 967 152 5 0 0 0 -3.143 0.885 0.932 4.911 2.819 0.343 -50
1.1.2 -0.40 130 2172 2158 590 181 196 88 100 100 0 2873 2929 547 1056 1065 132 100 100 0 1.096 1.056 1.083 1.053 1.341 1.055 0.856 0
1.2.2 -0.48 140 1675 1684 476 180 199 113 100 99 0 2332 2357 399 1045 1080 615 98 57 0 1.162 1.146 1.128 1.139 1.436 0.582 0.306 0
1.3.2 0.30 140 5112 5102 1416 183 207 119 100 100 0 4157 4245 1109 1040 1061 329 99 98 0 1.581 1.535 1.521 1.574 1.311 1.488 0.872 0
1.4.2 -0.04 140 3876 4027 1033 186 205 105 100 100 0 3972 4056 730 1035 1041 128 100 100 0 1.256 1.271 1.253 1.266 1.301 1.292 0.891 0

Total number of rows: 9216

Table truncated, full table size 1172 Kbytes.




Supplementary data files not provided
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