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Sample GSM19369 Query DataSets for GSM19369
Status Public on Jul 01, 2004
Title CEA_SGF:AHU027
Sample type RNA
 
Channel 1
Source name Reference 0h (Cy5)
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Tet OFF 48h (Cy3)
Organism Mus musculus
Extracted molecule total RNA
 
 
Description ARN ref 4x105 cells were plated in a 10 cm cell culture plate and cultured under standard condition (+LIF 1000 U/ml, tetracycline 1µg/ml) for 48h.
Tet OFF48h ARN test 4x105 cells were plated in a 10 cm cell culture plate and cultured in the presence of LIF 1000U/ml but without tetracycline for 48h to allow expression of STAT3F (Tet OFF 48h). Cells were then trypsinized and total RNA was extracted.
Lot batch = CEA_SGF:AHU
 
Submission date Mar 29, 2004
Last update date Oct 28, 2005
Contact name Dalila Sekkai
E-mail(s) dsekkai@igr.fr
Phone +33 (0)1 42 11 49 19
Organization name IGR
Department hematopoiesis and stem cell
Lab INSERM U362
Street address
City Villejuif
ZIP/Postal code 94805
Country France
 
Platform ID GPL1091
Series (1)
GSE1151 CEA_SGF:E00004#TetOFF_Stat3F

Data table header descriptions
ID_REF
VALUE log2 ratio of the median intensities of each feature for each wavelength, with the median background not subtracted.
DIA the diameter in µm of the feature-indicator.
F635_Median median feature pixel intensity at wavelength #1 (635 nm).
F635_Mean mean feature pixel intensity at wavelength #1 (635 nm).
F635_SD the standard deviation of the feature pixel intensity at wavelength #1 (635 nm).
B635_Median the median feature background intensity at wavelength #1 (635 nm).
B635_Mean the mean feature background intensity at wavelength #1 (635 nm).
B635_SD the standard deviation of the feature background intensity at wavelength #1 (635 nm).
%_>_B635+1SD the percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength #1 (635 nm).
%_>_B635+2SD the percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength #1 (635 nm).
F635_%_Sat the percentage of feature pixels at wavelength #1 that are saturated.
F532_Median median feature pixel intensity at wavelength #2 (532 nm).
F532_Mean mean feature pixel intensity at wavelength #2 (532 nm).
F532_SD the standard deviation of the feature intensity at wavelength #2 (532 nm).
B532_Median the median feature background intensity at wavelength #2 (532 nm).
B532_Mean the mean feature background intensity at wavelength #2 (532 nm).
B532_SD the standard deviation of the feature background intensity at wavelength #2 (532 nm).
%_>_B532+1SD the percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength #2 (532 nm).
%_>_B532+2SD the percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength #2 (532 nm).
F532_%_Sat the percentage of feature pixels at wavelength #2 that are saturated.
Ratio_of_Medians_(635/532) the ratio of the median intensities of each feature for each wavelength, with the median background subtracted.
Ratio_of_Means_(635/532) the ratio of the arithmetic mean intensities of each feature for each wavelength, with the median background subtracted.
Median_of_Ratios_(635/532) the median of pixel-by-pixel ratios of pixel intensities, with the median background subtracted.
Mean_of_Ratios_(635/532) the geometric mean of the pixel-by-pixel ratios of pixel intensities, with the median background subtracted.
Ratios_SD_(635/532) the geometric standard deviation of the pixel intensity ratios.
Rgn_Ratio_(635/532) the regression ratio of every pixel in a 2-feature-diameter circle around the center of the feature.
Rgn_R²_(635/532) the coefficient of determination for the current regression value.
Flags the type of flag associated with a feature.

Data table
ID_REF VALUE DIA F635_Median F635_Mean F635_SD B635_Median B635_Mean B635_SD %_>_B635+1SD %_>_B635+2SD F635_%_Sat F532_Median F532_Mean F532_SD B532_Median B532_Mean B532_SD %_>_B532+1SD %_>_B532+2SD F532_%_Sat Ratio_of_Medians_(635/532) Ratio_of_Means_(635/532) Median_of_Ratios_(635/532) Mean_of_Ratios_(635/532) Ratios_SD_(635/532) Rgn_Ratio_(635/532) Rgn_R²_(635/532) Flags
1.1.1 -1.76 140 411 442 190 170 194 220 52 16 0 1394 1453 335 905 921 160 88 72 0 0.493 0.496 0.496 0.483 2.386 0.383 0.411 0
1.2.1 -1.57 130 656 671 175 184 217 308 84 24 0 1954 1934 303 922 964 674 83 12 0 0.457 0.481 0.458 0.469 1.555 0.09 0.027 0
1.3.1 -1.15 130 1069 1136 414 185 208 128 100 100 0 2380 2337 505 934 982 676 91 57 0 0.611 0.678 0.645 0.674 1.615 0.632 0.647 0
1.4.1 -1.52 100 426 461 229 176 207 268 45 5 0 1219 1227 211 930 943 169 71 35 0 0.865 0.96 0.8 0.86 2.653 0.804 0.151 0
1.5.1 -1.68 150 602 639 214 179 201 112 98 87 0 1929 1952 307 947 959 142 100 98 0 0.431 0.458 0.446 0.43 1.747 0.42 0.553 0
1.6.1 -0.41 150 2534 2494 614 190 214 118 100 100 0 3369 3379 568 964 976 146 100 100 0 0.975 0.954 0.951 0.944 1.265 0.963 0.908 0
1.7.1 -1.64 130 503 585 807 185 204 107 85 70 0 1564 1586 264 981 981 128 99 90 0 0.545 0.661 0.545 0.525 2.365 8.296 0.077 0
1.8.1 -0.50 150 2765 2679 893 184 203 218 100 98 0 3915 3849 898 974 977 127 100 99 0 0.878 0.868 0.836 0.846 1.383 0.888 0.854 0
1.9.1 -2.36 160 186 198 81 178 198 204 2 0 0 954 967 115 979 983 117 15 1 0 -0.32 -1.667 0.571 0.587 3.691 10.191 0.022 -50
1.10.1 -2.41 160 182 193 93 179 195 98 10 1 0 968 971 125 983 986 127 13 1 0 -0.2 -1.167 0.623 0.635 4.736 2217.63 0 -50
1.11.1 -2.44 160 180 185 70 184 204 120 5 0 0 980 986 117 1020 1058 513 0 0 0 0.1 -0.029 0.505 0.51 4.113 0.04 0.003 -50
1.12.1 -2.57 160 197 216 109 189 208 126 10 3 0 1172 1179 167 1100 1141 511 1 0 0 0.111 0.342 0.482 0.487 3.881 0.543 0.117 -50
1.13.1 -2.56 160 181 195 72 182 223 516 0 0 0 1066 1079 158 1108 1125 159 8 1 0 0.024 -0.448 0.575 0.552 4.799 20.308 0.035 -50
1.14.1 -2.12 160 511 522 160 181 224 517 13 0 0 2218 2239 307 1138 1147 146 100 99 0 0.306 0.31 0.311 0.282 1.866 1.057 0.072 0
1.15.1 -3.31 160 339 348 127 187 200 86 67 42 0 3361 3385 433 1205 1213 143 100 100 0 0.071 0.074 0.079 0.071 1.996 0.066 0.327 0
1.16.1 -2.66 160 193 312 1216 189 303 1283 0 0 0 1224 1240 191 1255 1297 558 1 0 0 -0.129 -8.2 0.488 0.472 4.796 2.354 0.821 -50
1.1.2 -1.31 150 1027 1083 328 180 203 155 100 98 0 2546 2634 423 935 955 153 100 100 0 0.526 0.531 0.53 0.511 1.395 0.669 0.445 0
1.2.2 -1.25 160 854 866 261 183 207 269 95 69 0 2036 2035 321 951 959 136 100 99 0 0.618 0.63 0.616 0.611 1.504 0.759 0.372 0
1.3.2 -0.58 160 2989 2931 730 177 195 102 100 100 0 4455 4298 840 958 973 147 100 100 0 0.804 0.825 0.82 0.823 1.345 0.817 0.91 0
1.4.2 -0.75 160 1964 1921 619 180 202 110 99 98 0 3314 3219 741 965 981 171 100 98 0 0.759 0.772 0.77 0.762 1.585 0.759 0.858 0

Total number of rows: 3545

Table truncated, full table size 451 Kbytes.




Supplementary data files not provided
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