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Sample GSM1937021 Query DataSets for GSM1937021
Status Public on Apr 01, 2016
Title EV5
Sample type SRA
 
Source name MG1655 recN OD0,2 with MMC 20 min
Organism Escherichia coli str. K-12 substr. MG1655
Characteristics od: 0.2
strain: MG1655 recN
Growth protocol E. coli K12 MG1655 where grown in minimal medium A supplemented with casaminoacids 0,2% and glucose 0,25% until OD 0.2, treated or not with MMC (10µg/ml) for 20 or 40 min. Total RNA where immediatly extracted.
Extracted molecule total RNA
Extraction protocol Genejet RNA purification kit (Thermo)
Libraries were prepared according to Illumina's instructions accompanying the TruSeq SBS Kit Transcriptome . Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina Genome Analyzer
 
Data processing Basecalls performed using CASAVA-1.8.2 (demultiplexing), Fastqc 0.10.1 (read quality),
RNA-seq reads were aligned to the NC_000913 genome assembly using Rockhopper
Supplementary_files_format_and_content: mRNA profiling and RKPM were performed with Rockhopper
 
Submission date Nov 10, 2015
Last update date May 15, 2019
Contact name Olivier Espeli
E-mail(s) olivier.espeli@college-de-france.fr
Phone 33144271249
Organization name College de France
Department CIRB
Street address 11 place Marcelin Berthelot
City Paris
ZIP/Postal code 75005
Country France
 
Platform ID GPL17137
Series (1)
GSE74882 Genome-wide analysis of E.coli response to mitomycin (MMC) treatment in wild type and recN mutant
Relations
BioSample SAMN04254624
SRA SRX1426253

Supplementary file Size Download File type/resource
GSM1937021_EV5_transcripts.txt.gz 168.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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