|
| Status |
Public on Jul 05, 2018 |
| Title |
Tfh from TONS 1 [gene-level] |
| Sample type |
RNA |
| |
|
| Source name |
Ex-vivo Tfh cells
|
| Organism |
Homo sapiens |
| Characteristics |
pathology: Tonsillectomy
treatment: Untreated
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using AllPrep ARN/ADN kit (Qiagen, Valencia, CA)
|
| Label |
biotin
|
| Label protocol |
Biotinylated double strand cDNA targets were prepared with the NuGEN Ovation PicoSL WTA System V2 Kit followed by the NuGEN Encore Biotin Module Kitaccording to manufacturer recommendations
|
| |
|
| Hybridization protocol |
Following fragmentation and end-labeling, 4.7 μg of cDNAs were hybridized for 16 hours at 45°C on GeneChip® Human Transcriptome Array 2.0 (Affymetrix)
|
| Scan protocol |
GeneChips were scanned with the GeneChip® Scanner 3000 7G (Affymetrix) at a resolution of 0.7 µm
|
| Description |
Gene expression from tonsil CD4 Tfh cells
Tfh1.CEL
|
| Data processing |
Raw data (.CEL Intensity files) were extracted from the scanned images using the Affymetrix GeneChip® Command Console (AGCC) version 4.1.2. CEL files were further processed with Affymetrix Expression Console software version 1.4.1 using Robust Multi-array Average (RMA) algorithms with default settings at the gene and exon levels
|
| |
|
| Submission date |
Nov 13, 2015 |
| Last update date |
Jul 05, 2018 |
| Contact name |
Patricia Amé-Thomas |
| E-mail(s) |
patricia.ame@univ-rennes1.fr
|
| Organization name |
INSERM UMR1236
|
| Department |
Faculté de Médecine
|
| Street address |
2 Avenue du Pr Léon Bernard
|
| City |
Rennes |
| ZIP/Postal code |
35033 |
| Country |
France |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE74982 |
Expression data of T follicular helper (Tfh) and memory CD4 T cells from non malignant tonsils (TONS). |
|
| Relations |
| Alternative to |
GSM1939648 (exon-level analysis) |
