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Status |
Public on Jul 05, 2018 |
Title |
Memory T CD4 from TONS 1 [exon-level] |
Sample type |
RNA |
|
|
Source name |
Ex-vivo memory CD4 T cells
|
Organism |
Homo sapiens |
Characteristics |
pathology: Tonsillectomy treatment: Untreated
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using AllPrep ARN/ADN kit (Qiagen, Valencia, CA)
|
Label |
biotin
|
Label protocol |
Biotinylated double strand cDNA targets were prepared with the NuGEN Ovation PicoSL WTA System V2 Kit followed by the NuGEN Encore Biotin Module Kitaccording to manufacturer recommendations
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|
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Hybridization protocol |
Following fragmentation and end-labeling, 4.7 μg of cDNAs were hybridized for 16 hours at 45°C on GeneChip® Human Transcriptome Array 2.0 (Affymetrix)
|
Scan protocol |
GeneChips were scanned with the GeneChip® Scanner 3000 7G (Affymetrix) at a resolution of 0.7 µm
|
Description |
Gene expression from tonsil naive CD4 T cells N1.CEL
|
Data processing |
Raw data (.CEL Intensity files) were extracted from the scanned images using the Affymetrix GeneChip® Command Console (AGCC) version 4.1.2. CEL files were further processed with Affymetrix Expression Console software version 1.4.1 using Robust Multi-array Average (RMA) algorithms with default settings at the gene and exon levels
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Submission date |
Nov 13, 2015 |
Last update date |
Jul 05, 2018 |
Contact name |
Patricia Amé-Thomas |
E-mail(s) |
patricia.ame@univ-rennes1.fr
|
Organization name |
INSERM UMR1236
|
Department |
Faculté de Médecine
|
Street address |
2 Avenue du Pr Léon Bernard
|
City |
Rennes |
ZIP/Postal code |
35033 |
Country |
France |
|
|
Platform ID |
GPL17585 |
Series (1) |
GSE74982 |
Expression data of T follicular helper (Tfh) and memory CD4 T cells from non malignant tonsils (TONS). |
|
Relations |
Alternative to |
GSM1939639 (gene-level analysis) |