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Sample GSM194069 Query DataSets for GSM194069
Status Public on Dec 31, 2007
Title heart_control untreated rats
Sample type RNA
 
Source name heart
Organism Rattus norvegicus
Characteristics Sabra strain, males, 3 mo old, heart
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from individual tissue samples using Tri-Reagent isolation protocol. Three pools (6 animals) were prepared for each experimental group.
Label [32P]dATP
Label protocol Standard Clontech protocol was used
 
Hybridization protocol The membranes were prehybridized for 1 h at 68C in a hybridization solution (Clontech) containing 0.1 mg/ml sheared salmon testes DNA (Sigma) to block nonspecific binding. Synthesized radio labeled cDNA probe was applied to each membrane and hybridized overnight at 68°C. Cot-1 DNA (Clontech) (1 ug/ml) was added to block nonspecific binding.
Scan protocol For analysis, the membranes were exposed for 15 and 24 h to a phosphor screen and detected using a Bio-imaging Analyzer BAS2000 (Fuji Photo Film Co. Ltd Japan.
Description Gene expression data from left ventricle of rat heart
Data processing We used Atlas Image 2.01 software (Clontech) to grid the phosphor image, to record the pixel density of each spot, and to perform background subtraction. The background-subtracted data were then further analyzed.
 
Submission date May 24, 2007
Last update date Jun 11, 2007
Contact name Michal Horowitz
E-mail(s) horowitz@cc.huji.ac.il
Phone 972 2 6757588
Fax 972 2 6439736
Organization name The hebrew University
Lab Environmental Physiology
Street address POB 12272
City Jerusalem
ZIP/Postal code 91120
Country Israel
 
Platform ID GPL153
Series (1)
GSE7799 Cardiac and Soleus muscles following exposure of rats to heat acclimation and exercise training

Data table header descriptions
ID_REF 1
VALUE Normalized signal

Data table
ID_REF VALUE
R234 1.937
R077 0.308
R246 0.438
R024 0.131
RG068 0.177
RG076 0.217
RG146 2.327
RG396 0.164
RG481 0.076
R464 0.033
R215 0.208
R438 0.712
R496 0.213
RG007 1.3
RG299 0.243
R331 0.195
R221 0.072
R218 0.169
R038 0.054
R389 0.262

Total number of rows: 1185

Table truncated, full table size 13 Kbytes.




Supplementary file Size Download File type/resource
GSM194069.txt.gz 10.2 Kb (ftp)(http) TXT
Processed data included within Sample table

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