|
Status |
Public on Aug 18, 2017 |
Title |
ZR75-ERX-11-2 |
Sample type |
SRA |
|
|
Source name |
ZR75 breast cancer cells
|
Organism |
Homo sapiens |
Characteristics |
treatment: ESR1 Coregulator Binding Site Inhibitors tissue: ZR75
|
Treatment protocol |
Total RNA was isolated from ZR75 cells that are treated with vehicle or ERX-11 in the presence of E2 and Samples were subjected to RNAseq analysis
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA samples about 1-2 mg were used for sequencing library preparation according to Illumina TruSeq Total RNA Sample Preparation Guide (Illumina Cat. #: RS-122-2201). Each library was bar-coded and then pooled for cluster generation and sequencing run with 100bp single-end (SE) sequencing protocol.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
ZR75 cells treated with ERX+E2
|
Data processing |
Illumina Casava v1.8.2 software used for base-calling Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg19 whole genome using TopHat v2.0.8b gene count measurements were obtained using HTSeq-count Genome_build: hg19 Supplementary_files_format_and_content: The processed data files are in tabular format. The first column contains the gene symbols and the second column contain the read counts of the gene using htseq-count
|
|
|
Submission date |
Dec 03, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Yidong Chen |
E-mail(s) |
cheny8@uthscsa.edu
|
Phone |
2105629163
|
Organization name |
UT Health Science Center at San Antonio
|
Department |
Population Health Sciences
|
Street address |
8403 Floyd Curl Drive, MSC 7784
|
City |
San Antonio |
State/province |
Texas |
ZIP/Postal code |
78229 |
Country |
USA |
|
|
Platform ID |
GPL11154 |
Series (1) |
GSE75664 |
Estrogen receptor coregulator binding modulators (ERXs) effectively target estrogen receptor positive human breast cancers |
|
Relations |
BioSample |
SAMN04315698 |
SRA |
SRX1462168 |