|
| Status |
Public on Jun 30, 2016 |
| Title |
WBC_Air_Rep5 |
| Sample type |
RNA |
| |
|
| Source name |
white blood cells from male cynomolgous macaque collected immediately following the second 6-hour exposure to filtered air
|
| Organism |
Macaca fascicularis |
| Characteristics |
tissue: circulating white blood cells
gender: male
|
| Treatment protocol |
Animals were exposed, sedated, and euthanized using protocols approved by the Lovelace Respiratory Research Institute’s animal care and use committee.
A total of 14 primates received two consecutive days of 6-hour whole body inhalation exposures consisting of either filtered air (n = 6) or a target of 6 ppm formaldehyde (n = 8). To generate the exposure conditions, deuterated/13C labeled paraformaldehyde (Cambridge Isotope Laboratories, Inc) was vaporized and directed through a delivery line and into a Tedlar® bag. During each of the exposures, Tedlar® bags were diluted with pre-filtered air and delivered to the chambers. Chamber concentrations were monitored approximately twice per hour by collecting Waters XpoSure Aldehyde Sampler cartridges for five minutes. Cartridges were extracted with acetonitrile and analyzed by high-performance liquid chromatography. The average chamber formaldehyde concentrations during the exposure days ranged between 6.0 and 6.5 ppm.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Nasal epithelial tissue samples were disrupted and homogenized using a TissueRuptor (Qiagen) and RNA isolated using the AllPrep DNA/RNA/Protein kit (Qiagen). WBC samples were homogenized using QIAshredders (Qiagen) and RNA isolated using the AllPrep DNA/RNA/Protein kit. Extracted RNA was quantified with a Nanodrop 1000 spectrophotometer (Thermo Scientific, Waltham, MA) and its integrity verified with a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
terminal deoxynucleotidyl transferase (TdT) in the presence of a proprietary biotinylated compound, GeneChip® DNA Labeling Reagent
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on the Cynomolgus Gene 1.0 ST Array.
|
| Scan protocol |
Microarrays were scanned using Affymetrix Microarray Scanner.
|
| Description |
Gene expression data from circulating white blood cells of cynomologus macaques exposed to filtered air for 2-day, 6-hour whole body inhalation exposure
|
| Data processing |
Dana were normalized by robust multi-chip averging using Partek® Genomics Suite TM software (version 6.5)
|
| |
|
| Submission date |
Dec 07, 2015 |
| Last update date |
Jun 30, 2016 |
| Contact name |
Rebecca Catherine Fry |
| E-mail(s) |
rfry@unc.edu
|
| Organization name |
UNC-Chapel Hill
|
| Department |
Environmental Sciences and Engineering
|
| Street address |
1213 MHRC
|
| City |
Chapel Hill |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL21213 |
| Series (1) |
| GSE75759 |
Expression data from male cynomolgus macaques exposed to 6 ppm formaldehyde |
|
