|
| Status |
Public on May 27, 2008 |
| Title |
Fat line chickens, biological rep5 |
| Sample type |
RNA |
| |
|
| Source name |
fat line chicken adipose tissue at 7-week of age
|
| Organism |
Gallus gallus |
| Characteristics |
Strain: Arbor Acres breed,Gender:male
Age: 49 day after birth
|
| Treatment protocol |
Birds were slaughtered at 7 weeks and abdominal fat was isolated, immediately frozen in liquid nitrogen and stored at -80oC.
|
| Growth protocol |
All birds were kept in similar environmental conditions and had access to feed and water ad libitum, and were fed with a commercial corn-soybean-based diet that met all NRC requirements (National Research Council, 1994). From hatching to 3 weeks the birds received a starter feed (3,100 kcal ME/kg and 210 g/kg CP), and from 4 weeks to slaughter they were fed a grower diet (3,000 kcal ME/kg and 190 g/kg CP).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Chicken Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from chicken adipose tissue at 7-week of age
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jun 04, 2007 |
| Last update date |
May 27, 2008 |
| Contact name |
Hongbao Wang |
| Organization name |
Northeast Agricultural University
|
| Street address |
No. 59 Mucai Street, Xiangfang District
|
| City |
Harbin |
| ZIP/Postal code |
150030 |
| Country |
China |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE8010 |
Expression data from chicken adipose tissue at 7-week of age |
|
